C57BL/6J-HBV转基因小鼠随增龄乙肝表面抗原表达及转氨酶水平变化探讨

Study on Changes in HBsAg Expression and Glutamic-pyruvic Transaminase Levels with Age in C57BL/6J-HBV Transgenic Mice

目的探讨C57BL/6J-HBV乙肝转基因小鼠随增龄表面抗原表达及转氨酶水平的变化。方法经血清表面抗原检测,根据S/C.O值将乙肝转基因小鼠分为两组,两组小鼠在4～10周龄每隔一周以酶联免疫吸附法测定血清表面抗原水平;各组小鼠在5～11周龄每隔一周以全自动生化分析仪测定小鼠血清谷丙转氨酶(ALT)和谷草转氨酶(AST)水平。结果实验1组HBV乙肝转基因小鼠表面抗原表达6周龄与4周龄相比显著增高,6周龄至10周龄趋于稳定;实验2组的雄性小鼠表面抗原表达6周龄与4周龄相比极显著降低(P<0.01),而雌性小鼠在4周龄至10周龄表面抗原表达相对稳定。实验1组的雄性小鼠ALT水平9周龄与7周龄比较显著下降(P<0.05),其余小鼠在5周龄至11周龄呈现下降趋势,但无显著性差异。实验1组的雄性小鼠AST水平11周龄与7周龄相比显著升高(P<0.05)。结论乙肝表面抗原S/C.O值不同的转基因小鼠表面抗原表达随增龄变化趋势不同;血清谷丙转氨酶和谷草转氨酶水平在5～11周龄变化趋势相反。

Objective To investigate the changes in HBsAg expression and glutamic-pyruvic transaminase levels with age in C57BL/6J-HBV transgenic mice.Methods HBV transgenic mice were divided into two groups according to S/C.O value.Serum HBsAg were measured by using ELISA assay every other week from four-weeks to ten-weeks in all mice and the glutamic-pyruvic transaminase level was determined by automatic biochemical analyzer every other week from five-weeks to eleven-weeks old.Results HBsAg expression in six-week old mice went up markedly compared with four-week old individuals in the first group.HBsAg expression in six-week old male mice went down significantly（P〈0.01）in comparison to four-week olds in the second group while no obvious changes were observed from four-weeks to ten-weeks old in female mice of the same group.ALT levels of nine-week old male mice was lower（P〈0.05）than that of seven-week olds in the first group.AST levels of eleven-week old male mice was higher than that of seven-week old mice in the first group.ConclusionThe change in HBsAg expression was different because of different S/C.O values and variations in ALT and AST was reverse from five-week to eleven-week olds in HBV transgenic mice.