离子排斥色谱-直接电导检测法分析都柿酒中的有机酸

Separation and determination of organic acids by ion-exclusion chromatography with direct conductivity detection in the blueberry wine

研究离子排斥色谱-直接电导检测法分离测定都柿酒中多种有机酸。以Shim-pack SCR-102H柱为分离柱,确定最佳色谱条件为:2.0mmol.L-1 p-甲苯磺酸:乙腈(v/v,91/9)为流动相,流速1.0mL.min-1,柱温50℃,pH 2.8。对所测有机酸的检出限在0.06～1.05mg.L-1之间,相对标准偏差在3.3%以下(n=5),加标回收率在97.2%～103%之间。

A method was developed for the separation and determination of organic acids in the blueberry wine by ion-exclusion chromatography with direct conductivity detection.Chromatographic separation and determination of the acids was performed on a Shim-pack SCR-102H column.Excellent conditions for the separation and determination of the acids were achieved at 50 ℃,pH 2.8,1.0 mL·min-1 with the mixture of 2.0 mmol·L^-1 p-toluenesulfonic acid and acetonitrile（v/v,91/9） as eluent.The detection limits of the organic acids ranged from 0.06 to 1.05 mg·L^-1.The relative standard deviations for the organic acids were less than 3.3%（n=5） and the recoveries were from 97.2% to 103%.