摘要
初步探索乙醇-黄原胶耦联两步发酵的可行性和生产工艺,即控制乙醇发酵,发酵液去除固形物后蒸馏回收酒精,然后利用酒精糟液中剩余营养物质进行黄原胶发酵。摇瓶和小罐试验证实方案是可行的,耦联发酵工艺优化条件:选择木薯淀粉进行乙醇发酵60 h后的酒精糟液,添加2 g/L KH2PO4,不添加无机N源。在优化条件下,7 L发酵罐中耦联发酵的黄原胶产量达到20 g/L,转化率约为50%。
The feasibility and the technology of the ethanol-xanthan coupled two-stage fermentation were investigated.When ethanol fermentation was stopped ahead of time,the solid content was removed by centrifugation,the ethanol in the broth was recovered by distillation and the thin distiller with many nutrients was used for xanthan fermentation.The test in flask culture and 7 L batch fermentor proved the feasibility of the novel technology.The optimal conditions for the coupled fermentation were as follows:the thin distiller of ethanol fermentation(60 h) using cassava starch was chosen for xanthan fermentation,2 g/L of potassium dihydrogen phosphate was added into the medium and inorganic nitrogen source was not required.Under the above condition,20 g/L xanthan was obtained in 7 L fermentor with a yield of 50%.
出处
《生物加工过程》
CAS
CSCD
2011年第2期1-4,共4页
Chinese Journal of Bioprocess Engineering
基金
博士点基金新教师项目(20077434)
关键词
耦联两步发酵
乙醇
黄原胶
coupled two-stage fermentation
ethanol
xanthan gum