摘要
目的:检测Semaphorin(Sema)3A及其受体Neuropilin1(NRP1)在舌癌组织和舌鳞状细胞癌SCC9细胞株中的表达情况,同时观察外源性Sema 3A蛋白对舌鳞状细胞癌SCC9细胞株的影响。方法:通过细胞免疫化学、RT-PCR和Western blot-ting方法检测Sema 3A、NRP1在舌癌组织和SCC9细胞株中的表达情况;通过MTT实验检测外源性Sema 3A蛋白对SCC9细胞株增殖的影响;通过Transwell检测外源性Sema 3A蛋白对SCC9细胞株迁移、侵袭的影响。结果:Sema 3A在舌癌组织和SCC9细胞株中阴性表达,NRP1在舌癌组织和SCC9细胞株中阳性表达;100 ng/ml外源性Sema 3A蛋白明显抑制SCC9细胞株的增殖(P<0.05),对其迁移、侵袭能力影响未见统计学差异(P>0.05)。结论:舌癌组织和舌鳞状细胞癌SCC9细胞株中Sema 3A的阴性表达、NRP1的阳性表达可能与舌癌的发生发展有关。
Objective：To investigate the expression of Semaphorin（Sema） 3A and its receptor Neuropilin1（NRP1） in the human tongue squamous carcinoma tissues and cell line SCC9.The effect of exogenous Sema 3A protein on SCC9 was observed.Methods：Immunocytochemistry,reverse transcription polymerase chain reaction（RT-PCR） and Western blotting were used to detect the expression of Sema 3A,NRP1 in the human tongue squamous carcinoma tissues and cell line SCC9.MTT assay was used to study the effect of exogenous Sema 3A on the proliferation of SCC9.Transwell assay was used to investigate the effect of exogenous Sema 3A on the migration and invasion of SCC9.Results：The results of immunocytochemistry,RT-PCR and Western blotting indicated the negative expression of Sema 3A and positive expression of NRP1 in the human tongue squamous carcinoma tissues and cell line SCC9.The proliferation of SCC9 was inhibited by 100 ng/ml exogenous Sema 3A（P0.05）,but there was no difference in migration or invasion（P0.05）.Conclusions：There may be a close relationship between the negative expression of Sema 3A,positive expression of NRP1 and the process of tongue tumor generation and proliferation.
出处
《口腔生物医学》
2011年第1期21-25,共5页
Oral Biomedicine
基金
江苏省自然科学基金面上项目(42010005)
