烧伤早期及丹参对中性粒细胞化学发光的影响

Effect of burn patient's serum and Salvia miltiorrhiza on chemiluminescence magnitude of normal blood polymorphonuclear neutrophils

目的： 探讨烧伤早期患者血清（ＥＢＰＳ）调理的中性粒细胞（ＰＭＮ）对血管内皮细胞的损伤机制及丹参的保护作用，并对ＥＢＰＳ和丹参注射液（ＳＭ）调理ＰＭＮ的氧化活性及吞噬活性进行了研究． 方法： 以１００ ｍ Ｌ／ＬＥＢＰＳ， １００ ｍ Ｌ／ＬＥＢＰＳ加１０ ｍ Ｌ／ＬＳＭ 及１００ｍ Ｌ／Ｌ正常血清（ＮＨＳ）分别与健康人全血ＰＭＮ 孵育不同时间，检测ＰＭＮ 生物化学发光（ＰＭＮ－ＣＬ）． 结果： １００ｍ Ｌ／ＬＥＢＰＳ调理ＰＭＮ ０～１５ ｍ ｉｎ 与ＮＨＳ作用组比较，可促进ＰＭＮ－ＣＬ本底值和峰值；作用３０ｍ ｉｎ，两组无差异；作用１ ｈ，可抑制其本底值和峰值． ＳＭ 可明显降低ＰＭＮ－ＣＬ峰值． 结论： ＥＢＰＳ与ＰＭＮ作用，早期可激活ＰＭＮ 产生大量的氧自由基，进一步作用可抑制其氧化活性，由于早期烧伤血清调理ＰＭＮ可以产生大量的氧自由基，可能是造成血管内皮细胞损伤的重要原因，丹参可抑制其氧化活性．

AIM: To explore the injury mechanism that endothelial cells interact with stimulated polymorphonuclear neutrophils in early stage burn patient's serum (EBPS) and the protective effect of salvia miltiorrhiza, the chemiluminescence magnitude of stimulated PMN (PMN CL) was investigated. METHODS: Healthy whole blood interacted with100 mL/L EBPS, 10 per cent normal human serum (NHS) or 100 mL/L EBPS with 10 mL/L salvia miltiorrhiza injection (SM) at 37℃ respectively. The PMN CL of stimulated PMN was examined. RESULTS: When 100 mL/L EBPS was stimulated for 0 or 15 min, the background value and peak value of PMN CL increased, and when stimulated for 30 min, no statistical significance was observed in the background and peak value compared with NHS stimulated PMN. If PMN was further stimulated for 60 min, the peak value of PMN CL decreased significantly. 10 mL/L SM inhibited the peak value of EBPS stimulated PMN. CONCLUSION: The PMN, which is stimulated by EBPS, can produce a lot of oxygen free radical and may be a major reason of endothelial cell injury. Salvia miltiorrhiza can inhibit the oxidizing activity of PMN.