抗生素、亚硝基胍处理吸水链霉菌FC904原生质体对雷帕霉素产量的影响

Effects on the Production of Rapamycin by the Treatment of Protoplasts of Streptomyces hygroscopicus FC904 with Antibiotics and Nitrous Guanidine

目的 选择抗生素和化学诱变剂处理雷帕霉素产生菌的原生质体 ,筛选高产菌株。 方法 分别用庆大霉素、丝裂霉素 C、红霉素、亚硝基胍处理吸水链霉菌 FC90 4( S.hygroscopicus FC90 4)原生质体 ,比较它们对雷帕霉素产量的影响 ,并筛选高产菌株。 结果 庆大霉素的处理效果较好 ,从庆大霉素处理株中筛选到高产菌株 C14、C14 - 1,其雷帕霉素的摇瓶发酵水平 (反相高效液相色谱法测定 )分别比原始株提高 36% ,60 %。 结论 用庆大霉素处理吸水链霉菌 FC90 4原生质体是提高雷帕霉素产量的有效手段。

To screen higher producing strains by the treatment of protoplasts of S hygroscopicus FC904 with antibiotics and nitrous guanidine(NTG) \ Methods\ Protoplasts of S hygroscopicus FC904 were treated with gentamicin,erythromycin,mitomycin C or NTG respectively \ Effects of these agents on rapamycin production were compared and the high yielding strains were screened \ Results\ The treatment with gentamicin had a better effect on improving the production of rapamycin \ Higher producing strains C14 and C14 1 were obtained from the gentamicin treated strains with their RPM titer of broth(assayed by HPLC) increased by 36% and 60% respectively as compared with that of the original strain S hygroscopicus FC904 \ Conclusion\ The treatment of S hygroscopicus FC904 protoplasts with gentamicin is effective for improving the production of rapamycin \;