水稻红莲型不育系花药败育过程中线粒体亚显微结构的观察及ORFH79蛋白的亚细胞定位

Alstructure of mitochondria and immunogold localization of OrfH79 gene product in CMS-HL rice

为了了解水稻红莲型细胞质雄性不育系小孢子发育过程中线粒体的结构特征以及水稻红莲型细胞质不育系基因orfh79蛋白的亚细胞的定位,利用透射电镜分别观察了红莲型性不育系水稻小孢子发育过程中花粉母细胞,四分体和单核期三个发育时期花药线粒体的超微结构的变化,并结合胶体免疫金电镜技术研究了orfh79蛋白的亚细胞的定位。结果表明,与育性正常的保持系(YtB)同期小孢子发育相比,水稻红莲型不育系(YtA)小孢子发育的四分体和单核花药期的线粒体的结构出现了异常。同时,胶体免疫金电镜结果表明orfh79蛋白集中分布于不育系花药的线粒体中。上述实验结果从细胞学角度为深入研究红莲型细胞质雄性不育的机理奠定了基础。

Alstructre of mitochondrial of anther at various stages of development in YTA line were analyzed,meanwhile,the localization of ORFH79 protein were assayed using electron microscope immunocytological.In YTA line,the mitochondria becomes almost completely disintegrated,with disorganized cristae and a diffuse matrix,whereas those of YtB were normal during microgenesis.orfh79 protein was found mainly localized in the mitochondria of anthers in YtA.