摘要
以果蝇(Drosophila melanogaster)为试验动物,探讨亚硝酸钠对果蝇寿命、超氧化物岐化酶(SOD)活性及丙二醛(MDA)含量的影响。结果表明:培养基中添加亚硝酸钠能显著缩短果蝇平均寿命,其中30 g/L、120 g/L和480 g/L组雌果蝇平均寿命与对照组相比分别缩短了12.53、31.49、48.79 d,雄果蝇平均寿命与对照组相比分别缩短了3.15、12.834、4.11 d。饲喂亚硝酸钠后果蝇体内的SOD活性降低,MDA含量增加,与对照组相比,用亚硝酸钠30 g/L1、20 g/L和480 g/L饲喂果蝇10 d和20 d时,雌性果蝇体内总SOD活性分别降低了8.52%、31.06%、41.69%和14.79%、34.64%、47.78%,MDA含量分别增加了26.94%、48.71%、72.02%和20.63%、40.56%、83.92%,均达到了显著或极显著水平(P<0.05或P<0.01);雄性果蝇体内SOD活性分别比对照降低9.47%、34.13%、44.62%和11.18%、31.66%、46.10%,MDA含量分别增加了21.74%、48.40%、74.46%和24.91%、45.67%、74.39%。除饲喂30 g/L亚硝酸钠的雄蝇处理组在10 d时SOD活性与对照差异不显著外,其他处理均达到了显著或极显著水平(P<0.05或P<0.01)。
In this study,Drosophila melanogaster was used as the experimental animal to study the effects of sodium nitrite on life-span,superoxide dismutase(SOD) activity and malondialdehyde(MDA) content in D.melanogaster.The results showed that sodium nitrite significantly shortened drosophila average life-span.The decreased average life-span of female D.melanogaster was 12.53,31.49 and 48.79 days when the concentrations of sodium nitrite were 30,120 and 480 g/L.Average life-span of the male was shortened 3.15 d,12.83 and 44.11 days,respectively.After cultured with the sodium nitrite,the activities of the SOD decreased and MDA content increased.Compared to the control group,the activities of the SOD of female D.melanogaster decreased 8.52%,31.06% and 41.69% or 14.79%,34.64%,and 47.78%(P0.05 or P0.01) when D.melanogaster was fed with sodium nitrite at the concentration of 30,120 and 480 g/L for 10 d and 20 d,while the MDA content increased 26.94%,48.71%,72.02% and 20.63%,40.56%,83.92%(P0.05 or P0.01).The activities of the SOD of male D.melanogaster decreased 9.47%,34.13%,44.62% and 11.18%,31.66%,46.10% while MDA content increased 21.74%,48.40%,74.46% and 24.91%,45.67%,74.39%.Compared to the control group,SOD activity and MDA content significantly increased in sodium nitrite(P0.05 or P0.01) except for SOD activity of 30 g/L sodium nitrite experimental group after male D.melanogaster fed 10 days.
出处
《河南农业科学》
CSCD
北大核心
2011年第2期149-152,共4页
Journal of Henan Agricultural Sciences
基金
河北大学青年基金项目
保定市科技局项目(10ZF002)