摘要
樱桃叶斑驳病毒(Cherry mottle leaf virus,CMLV)是南美洲樱桃上重要的病害之一。本研究针对Genbank上公布的该病毒的核酸序列,人工合成了CMLV(AF170028.1)6741～7322 nt的核酸序列并连接载体,构建了阳性标准质粒,进行实时荧光定量PCR(Fluorescence quantitative,FQ-PCR)检测。实验结果表明,本研究设计的利用FQ-PCR检测CMLV的引物及探针特异性良好,经灵敏度检测,最低检测浓度为23 copies/μL,比普通PCR检测灵敏度高100倍。该FQ-PCR检测方法为樱桃叶斑驳病毒的防控提供了重要的技术支持。
Cherry mottle leaf virus（CMLV） is one of the most important diseases on cherry in South America.In this research,we successfully synthesized the sequences of CMLV 6741~7322 nt genes（Genbank AF170028.1） and constructed positive standard plasmids of this nucleic fragment on the vector pBluescript II SK（＋）.According to the result of the real-time PCR,23 copies/μL of the plasmid was detected as the detection limit of the real-time PCR,and 100 times more sensitive than conventional PCR.Therefore,the FQ-PCR method provides an important technical support for the detection and prevention of Cherry mottle leaf virus.
出处
《植物检疫》
北大核心
2011年第2期28-31,共4页
Plant Quarantine
基金
公益性行业科研专项项目(200810632)
