利用条形码技术鉴定苹浅褐卷蛾

Identification of Epiphyas postvittana for biosecurity using DNA barcoding.

苹浅褐卷蛾原产澳大利亚,目前仅分布于少数地区,是澳大利亚、新西兰及英国等国家多种经济作物上的重要害虫,苹浅褐卷蛾形态具有多态性,对其形态鉴定一直非常困难,由此也增加了该虫在口岸上的入侵风险。本研究使用DNA条形码技术(barcoding)测定了采集自新西兰5个不同地区苹浅褐卷蛾种群26个样本的细胞色素酶I(COI)序列。研究表明,苹浅褐卷蛾种内COI序列差异远小于3%,而与BOLD数据库中卷蛾科其他种类的种间差异约为7%～16%,远大于3%。该结果肯定了条形码技术能够成为口岸快速、准确鉴定苹浅褐卷蛾的有效方法。

Light brown apple moth（LBAM）,Epiphyas postvittana,is a leafroller pest native to Australia and currently has limited distribution around the world.As a serious pest in its current distribution areas,it is well-known for its polymorphism with respect to its morphology.Morphological identification of E.postvittana has been problematic which increased the risk of it esacaping detection at the border of countries that wish to regulate this pest.In this study,we sequenced the COI gene from 26 samples of E.postvittana from five populations in New Zealand.We found that the intraspecies variation of E.postvittana is less than 3%,while interspecies variation between E.postvittana and other tortricid species available in the barcode of life database（BOLD） system is much greater than 3%.This result confirms that using barcodes for identification of E.postvittana for biosecurity purposes is practical.