顶空气相色谱法测定人参根皂苷中的大孔吸附树脂残留物

Determination of Macroporous Resin Residua in Saponins of the Roots of Panax ginseng C A Meyer by Headspace-GC

采用顶空气相色谱法测定人参根皂苷中大孔树脂残留物的质量浓度.色谱柱初始温度60℃,保持22 min,以50℃/min升至200℃,保持6 min,氢火焰离子检测器温度为260℃,进样口温度220℃;用质量分数为40%的N,N-二甲基甲酰胺作溶剂,载气为氮气.实验结果表明,正己烷、苯、甲苯、对二甲苯、苯乙烯、对二乙基苯6种大孔树脂残留物在各自的质量浓度范围内呈良好的线性关系,且回收率符合要求.

To establish headspace-GC method for the determination of residua of macroporous resin in the saponins extracted from the roots of Panax ginseng C A Meyer,the chromatographic separation was performed.The column temperature was programed,with an initial temperature of 60 ℃（hold 22 min）,at an increasing rate of 50 ℃/min to 200 ℃（hold 6 min）;the injector temperature was set at 220 ℃ and FID detector temperature was set at 260 ℃;solvent was 40% N,N-Dimethyl acetamide（DMF）;the carrier gas was nitrogen.All the residua including n-hexane,benzene,toluene,1,4-xylene,styrene and 1,4-diethyl-benzene gave good linearities and the recoveries.