益气生骨颗粒提取液对体外成纤维细胞增殖的影响

Study on Promoting Fibroblasts,proliferation with "Reinforcing Qi and Producing Bone" Granules

目的:评价不同浓度益气生骨颗粒提取液对成纤维细胞增殖的影响。方法:采用体外培养9 d鸡胚皮肤的成纤维细胞,在成纤维细胞培养液中分别加入不同浓度的益气生骨颗粒提取液(简称益气)、黄芪注射液(简称黄芪)、复方当归注射液(简称当归),应用倒置显微镜每日观察细胞的生长情况和计数单位面积内的细胞数测定1-7 d的细胞增殖动力学。结果:各种药物对成纤维细胞生长的最佳浓度为:益气,0.25 mL%;黄芪,2 mL%;当归,0.125 mL%。与空白组(0%)相比,0.25 mL%益气组与2 mL%黄芪组在第3、5、7天的成纤维细胞均明显增多(均P<0.05);0.125 mL%当归组的细胞计数差异无统计学意义(P>0.05)。结论:益气生骨颗粒提取液能促进成纤维细胞增殖,0.25ml%是其最佳浓度。

Objective：To evaluate the effect of proliferation of fibroblasts with extracting solution of ＂Reinforcing Qi and Producing Bone＂ Granules（RQPBG）.Methods：The fibroblasts of chick embryo＇s skin of 9 days were isolated and cultured.After being stimulated by extracting solution of RQOB,the astragalus injection and the compound angelica injection with different concentration,growth and proliferation dynamics of fibroblasts from 1st to 7th day were determined by the count of the cells in a unit area every day from converting the discrepancy microscope＇s looking glass.Results：The best concentration of every liquor for the growth of fibroblasts：extracting solution of RQPBG,0.25ml%;the astragalus injection,2ml%;the injection of compound angelica,0.125ml%.Compared with the blank group,the count of fibroblasts on the 3th,5th,7th day,have evidently increased in the 0.25ml% RQPBG and the 2ml% astragalus;the count of fibroblasts have no significantly difference in the 0.125ml% angelica.Conclusions：Extracting solution of ＂Reinforcing Qi and Producing Bone＂ Granules can promote the fibroblasts＇ multiplication.The best concentration is 0.25ml%.