经皮冠状动脉腔内成形术对冠心病患者外周血白细胞膜表面粘附分子表达的影响

Effect of Percutaneous Transluminal Coronary Angioplasty on Expression of Adhesion Molecule on Leukocytes in Patients with Coronary Artery Disease

目的：探讨经皮冠状动脉腔内成形术（ＰＴＣＡ）后白细胞膜表面粘附分子表达的变化。 方法：以行常规冠状动脉造影的１１ 例冠心病患者为对照组，２１ 例行单支冠状动脉选择性ＰＴＣＡ 冠心病患者为ＰＴＣＡ组；用流式细胞仪直接免疫荧光法检测术前、术后３０ 分钟中性粒细胞和单核细胞膜表面ＣＤ１１ｂ、ＣＤ４９ｄ 和ＣＤ５４表达。 结果：ＰＴＣＡ后中性粒细胞和单核细胞膜表面ＣＤ１１ｂ 及ＣＤ４９ｄ 表达明显升高（Ｐ均＜ ０．０１），而ＣＤ５４ 表达无明显变化；对照组冠状动脉造影前后细胞粘附分子表达无显著差异。 结论：ＰＴＣＡ可使冠心病患者外周血中性粒细胞和单核细胞激活，并上调粘附分子ＣＤ１１ｂ 及ＣＤ４９ｄ

Objective:To investigate changes in the expression of adhesion molecule on leukocytes in patients with coronary artery disease(CAD) after percutaneous transluminal coronary angioplasty(PTCA). Methods:The expression of adhesion molecule CD11b,CD54 and CD49d on neutrophils and monocytes were measured by direct immunofluorescence techniques associated with flow cytometry before and 30 min after PTCA in 21 patients undergoing elective coronary angioplasty,11 CAD patients who underwent coronary arteriography were served as control group. Result:After PTCA,there were an increase in neutrophils and monocytes CD11b and CD49d expression( p <0 01),but no changes in CD54 expression.In the control subjects,no changes in adhesion molecule expression occurred. Conclusion:These findings suggest that PTCA triggers leukocytes activation in circulation and upregulates adhesion molecule CD11b and CD49d expression.