运动训练对去势后雌鼠骨密度及血清生化指标的影响

Effects of movement exercise on the bone mineral density of lumbar vertebra and serum biochemical indexes in ovariectomized rats

目的探讨运动训练对于预防绝经后骨质疏松的生物学作用。方法选取雌性SD大鼠45只,随机分为正常对照组、单纯去势组以及去势+运动训练组3组,正常对照组行假手术,剩余2组行卵巢切除术。正常对照组、单纯去势组自由活动,去势+运动训练组术后每日行跑步训练。术后12周分别做所有动物的骨组织病理学检测,测量腰椎骨密度以及血清中钙磷离子、碱性磷酸酶和骨钙素水平。结果腰椎骨密度测定显示正常对照组及去势+运动训练组明显高于单纯去势组;血清中生化指标检测显示正常对照组及去势+运动训练组钙离子、碱性磷酸酶和骨钙素水平均明显低于单纯去势组;腰椎椎体组织病理学检测显示去势+运动训练组骨小梁质量优于单纯去势组。结论运动对于预防和治疗绝经后骨质疏松有着很好的效果。

Objective To detect the bone tissue in lumbar vertebra histologically, the levels of serum biochemical indexes as well as bone mineral density, and to explore the biological effects of movement exercise against osteoporosis. Methods Forty five female rats were divided into three groups randomly： normal control group, simple ovarieetomized group and ovarieetomized＋training group. Animals in normal control group were sham operated on, and animals in other two groups were subjected to ovariectomy. Animals in normal control group and simple ovarieetomized group were allowed unrestricted activity in the cage after operation, while animals in ovarieetomized＋training group were trained running every day; all the rats were killed twelve weeks after the operation, and the bone tissue in lumbar vertebra were observed histologically, bone mineral density and serum biochemical indexes （Ca2＋, P5＋, ALP, BGP） were detected. Results Bone mineral densities of lumbar vertebra in normal control group and ovarieetomized＋training group were obvious higher than that in simple ovarieetomized group; Serum levels of Ca2＋, P5＋, ALP and BGP in normal control group and ovarieetomized＋training group were obvious lower than that in simple ovarieetomized group; Quality of bone trabecula in ovarieetomized＋training group was better than that in simple ovarieetomlzed group. Conclusion Movement exercise has good effect on precaution and treatment of osteoporosis.