摘要
分子量是蛋白质主要的特征参数之一,近年来其测试方法发展十分迅速。该文概述了目前蛋白质分子量测试中应用最广泛的凝胶电泳法、高效凝胶过滤色谱-激光光散射法以及生物质谱技术的原理和应用发展现状等。
Molecular weight is one of the most important characteristic parameters of proteins,which leads the methods to determine protein molecular weight to develope rapidly in recent years.In this paper,the mechanism,application and development were briefly overviewed for the most widely used technologies including gel electrophoresis,high performance gel filtration chromatography-laser light scattering and the biological mass spectrometry technology.
出处
《中国测试》
CAS
2011年第2期34-37,共4页
China Measurement & Test
关键词
蛋白质
分子量
凝胶电泳
高效凝胶过滤色谱-激光光散射
生物质谱
protein
molecular weight
gel electrophoresis
high performance gel filtration chromatography-laser light scattering
biological mass spectrometry