摘要
目的:研究AB-8大孔树脂并聚酰胺柱层析纯化瘤果黑种草子总黄酮工艺。方法:用紫外分光光度法测定瘤果黑种草子总黄酮含量,以总黄酮洗脱量为指标,筛选AB-8大孔树脂纯化工艺。结果:合适的上样条件:pH值为3,上样液浓度为4.90mg·mL^(-1),吸附流速为1BWh,洗脱剂为30%乙醇。结论:AB-8大孔树脂并聚酰胺树脂适合于瘤果黑种草子总黄酮的分离纯化。
Objective： To study technique condition for separating total flavones from Nigella Glandulifera by AB- 8 macroporous adsorption resin and polyamide column chromatography. Method： The contents of flavones was detenTtined by ultraviolet spectrophotometry. By the quantity analysis of eluted flavones, condition of purification was selected with AB - 8 macroporous adsorption resin. Results： Optimal purification condition of flavones was achieved with the sample pH of 3, total flavones concention in the solution of 4.90 mg·mL^-1 sample flow velocity of 1BV/h, and washing with 30% ethanol. Conclusion： AB- 8 macropomus adsorption resin and polymaide column chromatography can be well applicable for enrichment of flavones in Nigella C.landulifera.
出处
《中国民族医药杂志》
2011年第2期65-67,共3页
Journal of Medicine and Pharmacy of Chinese Minorities
基金
国家自然科学基金项目(30960512)
关键词
瘤果黑种草
总黄酮
AB-8大孔树脂
纯化
Nigella Glandulifera
total flavones
AB - 8 macropomus resin
purification
