摘要
用常规海马脑片实验方法,撤除人工脑脊液中的Mg2+,0.5h内CAl区出现自发性痫样放电,1h内趋于稳定,约1/3脑片波幅可稳定2h左右,但放电频率呈缓慢增加。无Mg2+灌流后1~3h间可以观察抗病药的作用。苯受英钠(PHT)100μmol/L,氯甲酸氮卓(CBZ)100μmol/L,安定(DZP)30μmol/L,苯巴比妥钠(PB)100μmol/L,而戊酸钠(VPA)10mmol/L见及乙琥胺(ESX)10mmol/L均可抑制波幅,量效关系明显。对频率和放电时程的抑制继发于波幅抑制,且缺乏量效关系。此模型可用来筛选广谱抗病药物,具有简便易行的优点,但不适合于长时间实验观察。
When rat hippocampal slices were perfused with Mg2+-free artificial cerebrospinal fiuid, spon-taneous epileptic discharges (SED) at CAl region could be recorded within 0.5h and became stablewithin lh. Although the frequency thereafter increased gradually, the amplitude maimeined stablefor 2h in about 1/3 of slices, which allowed a 2h observation for the action of anticonvulsants.Phenytoin (PHT 100 μmol/L), carbamazepine (CBZ 100 μmol/L), diazepam (DZP 30μmol/L),sodium phenobarbital (PB 100μmol/L), sodium valproate (VPA lOmmol/L) and ethosuximide (ESXl0mmol/L) suppressed the amplitude of SED with a good concentration-response relation. The sup-pressi0n of frequency and duration of SED depended on the suppression of amplitude and lackedconcentration-response relation. The results suggest that this model is simple and easy to be used inscreening broadspectrum anticonvulasants, but is not suitable for long term observation.
出处
《基础医学与临床》
CSCD
1999年第5期51-55,60,共6页
Basic and Clinical Medicine
基金
北京市自然科学基金
北京市科技新星计划资助!7962006