听力对噪声的易感性与线粒体DNA7.4kb缺失的初步观察

Observation on susceptibility of hearing to noise exposure and mitochondrial DNA 7.4kb deletion

目的 探索线粒体基因突变与个体听力对噪声易感性差异的关系。方法 采用ＰＣＲ 扩增技术检测线粒体ＤＮＡ（ｍｔＤＮＡ）７ ．４ ｋｂ 的缺失。结果 在２１ 名听力易感工人和２０ 名非易感工人的ｍｔＤＮＡ 缺失常发区８６３７ ～１６０７３ 之间共检测到４ 种不同的缺失，对应缺失长度在３ ．１ ～７ ．４ ｋｂ 之间。易感组７ ．４ ｋｂ 缺失率（１０／２１） 明显高于对照组（３／２０） ，ＯＲ＝ ５ ，Ｐ＜ ０ ．０５ 。结论 ｍｔＤＮＡ７ ．４ ｋｂ

Objective To explore the relation between susceptibility of hearing to noise exposure and mitochondrial DNA (mtDNA) deletion. Methods The DNA was collected from white blood cells of 41 workers.Twenty one of them were susceptible to noise exposure and the other twenty were not susceptible.An advanced PCR technique was applied to observe the 7.4 kb mtDNA deletion. Results Four kinds of deletion were found from 3.1 kb to 7.4 kb in these workers.The 7.4 kb deletion rate in susceptible group(10/21) was significantly higher than that in control group (3/20), OR =5, P <0.05. Conclusion The 7.4 kb mtDNA deletion is one of the possible risk factors for individual susceptibility of hearing to noise exposure.