T-RFLP分析技术在肉鸡肠道微生物研究中的应用

Application of T-RFLP Technique in the Studying of Gut Microflora in Broilers

本文阐述了末端限制性片段长度多态性(T-RFLP)分析技术的基本原理和技术路线,讨论了应用T-RFLP分析技术相对于应用传统方法研究肉鸡肠道微生物的优势,总结了应用T-RFLP分析技术研究肉鸡肠道微生物的发展历程,分析了应用T-RFLP分析技术研究肉鸡肠道微生物存在的问题及解决方法,并对其应用前景进行了展望。

The basic principles and technical route of T-RFLP（terminal-restriction fragment length polymorphism）were introduced in this paper,the advantages of T-RFLP over traditional research methods for studying intestinal microflora of broilers were discussed.Meanwhile,the development of the application of T-RFLP technique for studying microflora in the broiler gut and its problems and solutions were also summarized in this paper.