延胡索中延胡索乙素双相动态提取方法研究

Diphasic dynamic extraction method of tetrahydropal matine in corydalis

目的探讨延胡索有效成分的最佳双相动态提取工艺。方法以延胡索乙素含量为指标,采用L9(34)正交试验设计对提取工艺进行优化,考察酸浓度、溶剂倍数、提取时间、提取温度对延胡索乙素含量的影响。结果延胡索乙素的最佳提取工艺为加酸浓度2.0%的硫酸,10倍量溶剂,100℃提取1次,提取时间3 h。结论优选得到的工艺确实可行,便于生产质量控制。

Aim To establish the optimal diphasic dynamic extraction preparation procedure of active ingredients in corydalis.Methods The influential factors for the extraction of active ingredients in corydalis included acid concentration,solvent amount,time of extraction,extraction temperature.The extraction technology was optimized by orthogonal test.Results The most favorable extraction conditions were as follows：adding 2.0%sulfate of 10 times the quantity of medicinal materials into Chinese medical materials and boiling for 3h with the tempreture of 120℃.Conclusion This technological process is feasible and can be used to control the quality of corydalis.