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鲫鱼一种新型SNRPC基因的蛋白表达和抗体制备分析 被引量：2

A New Type SNRPC Protein Expression and Preparation of Antibody in Carassius carassius gibelio
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摘要 本研究构建了鲫鱼卵母细胞特异表达的新型SNRPC基因的原核表达载体,表达并纯化蛋白,制备多克隆抗体,并检测抗体特异性,为进一步研究新型SNRPC基因的功能奠定基础。首先设计引物,应用RT-PCR从鲫鱼成熟卵母细胞中获得含该基因片段,重组入原核表达载体PinPoint T中,在大肠杆菌BL-21(DE3)中诱导表达,应用亲和层析法获得纯度较高的原核表达蛋白并免疫家兔制备多克隆抗体,用Western blot方法检测此抗体特异性。成功构建了新型SNRPC基因重组表达载体,表达的蛋白经纯化后免疫家兔得到了特异的多克隆抗体。鲫鱼卵母细胞的新型SNRPC基因的原核表达载体的构建、重组蛋白的表达纯化及抗体的制备为后续的研究提供了理论基础。 The prokaryotic expression vector of differentially expressed gene SNRPC in Carassius carassius was constructed.The recombinant protein was purified to prepare polyclonal antibody.A gene fragment was amplified from cDNA of mature oocyte of crucian carp,inserted into PinPoint T vector and introduced into BL-21（DE3） strain.The recombinant protein was purified by affinity chromatography and then generated the polyclonal antibody with identification by Western blot method.The specific polyclonal antibody was obtained,and it would be used to the functional assay.
作者 王红莹 杜嫚
机构地区 中南民族大学生命科学学院
出处 《河北农业科学》 2010年第12期43-45,共3页 Journal of Hebei Agricultural Sciences
关键词 鲫鱼 新型SNRPC 蛋白表达 多克隆抗体 抗体 制备 Carp New type SNRPC Protein expression Polyclonal antibody Antibody Preparation
分类号 Q786 [生物学—分子生物学]
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参考文献10

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同被引文献10

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河北农业科学
2010年 第12期

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