BEAC工艺中微生物群落变化和种群稳定性

Analysis of microbial community dynamics and population stability in BEAC procedure

为了解生物增强活性炭(BEAC)上微生物群落变化和种群的稳定性,从运行13 d、26 d和39 d的BEAC炭柱的上层、中层、下层进行取样,通过细胞裂解来获取基因组DNA,纯化后,用对细菌16S rDNA基因V3区具有特异性的通用引物F357-GC和R518对其进行聚合酶链式反应(PCR)扩增,得到长约250 bp的PCR产物.用变性梯度凝胶电泳(DGGE)对PCR产物进行分离,获得炭柱内微生物群落的16S rDNA基因V3区的指纹图谱.研究表明:随着反应器的运行,活性炭柱内微生物的群落结构、种类以及数量都具有时序动态性;原水带入的其他菌的种类和数量不断减少;人工强化固定的5株菌一直存在,它们在空间上呈现不同的分布,这些菌经历了动态的演替后,逐渐成为优势菌群,群落结构趋于稳定.固定化菌群的稳定性从根本上保证了整个系统的运行和处理效果的稳定性.

To study microbial community dynamics and population stability on the bioenhancement activated carbon（BEAC）,the samples were taken from different position of the carbon column at different operation time.The Genomic DNA of the microbes on the activated carbon was released by breaking the bacterial cells.After purification of the genomic DNA,the V3 region of genomic 16S rDNA was amplified by using specific primers（F357-GC and R518）.The polymerase chain reaction（PCR）products were about 250 bp and then separated by parallel denaturing gradient gel electrophoresis（DGGE）.The profile of DGGE showed that the structure,categories and number of microbial community were changing dynamically during running of the reactor.The categories and number of the inbreaking bacteria were reducing continuously.5 artificial enhanced and immobilized bacteria were existing all through and distributed differently in the carbon column.Further more,they became dominant community after dynamic succession and community structure tended to be stable.The stability of the immobilized bacteria ensured the stability of operation and removal effect of BEAC procedure.