期刊文献+

两株抗氧化活性酵母的鉴定及对乳成分的影响

Identification of two yeast stains with antioxidant activity and their effects on the main component of bovine milk
下载PDF
导出
摘要 为获得有抗氧化活性的工业用酵母菌株,对2株来源于原料乳中有较强抗氧化活性的酵母菌进行鉴定.根据菌株的表型、生理生化特征和基因型的特性,初步将这2株菌鉴定为毕赤氏发酵酵母(Pichia fermentans).将这2株酵母菌在高温灭菌(UHT)牛乳中培养以研究其对牛乳主要成分的影响,结果表明:这2株酵母能在UHT牛乳中产乙醇,具有促进蛋白水解活性与脂肪水解活性的能力,能有效提高乳中的游离氨基酸和脂肪酸的含量. To obtain industrial yeast strains with antioxidant activity,identification of two yeasts with excellent antioxidant activity,which were isolated from raw milk,were carried out in this paper.Based on their morphological,biochemical,physiological and genotypic characterization,two strains were identified as Pichia fermentans.Then,the influence of these two strains on the main components in the milk was further evaluated by incubating in ultra high temperature(UHT)-processed bovine milk.The results indicated that both of these two strains were able to produce ethanol and facilitate proteolysis and lipolysis,and increased the content of free amino acid and fatty acid in the UHT milk.
出处 《哈尔滨工业大学学报》 EI CAS CSCD 北大核心 2010年第12期1932-1937,共6页 Journal of Harbin Institute of Technology
关键词 PICHIA fermentans 鉴定 乳糖发酵 蛋白水解活性 脂肪水解活性 Pichia fermentans identification lacto fermentation proteolysis lipolysis
  • 相关文献

参考文献19

  • 1FLEET G H. A Review: Yeasts in dairy products [ J ]. Journal of Applied Bacteriology, 1990, 68:199-211.
  • 2ROHM H, ELISKASES- LECHNER F, BRUER M. Diversity of yeasts in selected dairy products [ J ]. Journal of Applied Bacteriology, 1992, 72 : 370 - 376.
  • 3DEAK T, BEUCHAT L R, GUERZONI M E, et al. A collaborative study on media for the enumeration of yeasts in foods [ J ]. International Journal of Food Microbiology, 1998, 43 : 91 -95.
  • 4ROOSTITA R, FLEET G H. Growth of yeasts in milk and associated changes to milk composition [ J ]. Inter- national Journal of Food Microbiology, 1996, 31 : 205 - 219.
  • 5PSOMAS E I, FLETOURIS D J, LITOPOULOU- TZANETAKI E, et al. Assimilation of cholesterol by yeast strains isolated from infant feces and feta cheese [ J ]. Journal of Dairy Science, 2003, 86: 3416 - 3422.
  • 6KUMURA H, TANOUE Y, TSUKAHARA M, et al. Screening of dairy yeast strains for probiotic applica- tions [ J]. Journal of Dairy Science, 2004, 87:4050 - 4056.
  • 7ELMER H M, JAMES L S. Applied Dairy Microbiology [ M ]. New York : Marcel Dekker, 2001.
  • 8DE FREITAS I, PINON N, MAUBOIS J L, et al. The addition of a cocktail of yeast species to Cantalet cheese changes bacterial survival and enhances aroma compound formation [ J ]. International Journal of Food Microbiology, 2009, 129 : 37 - 42.
  • 9GORI K, MORTENSEN H D, ARNEBORG N, et al. Ammonia production and its possible role as a mediator of communication for Debaryomyces hansenii and other cheese-relevant yeast species [ J ]. Journal of Dairy Science, 2007, 90:5032-5041.
  • 10WANG S Y, CHEN H C, LIU J R, et al. Identification of yeasts and evaluation of their distribution in Taiwan Residents kefir and viili starters[ J ]. Journal of Dairy Science, 2008,91 : 3798 - 3805.

二级参考文献10

  • 1NAYLIN N, TAING O, HASHINAGA F, et al. Antioxidant activity of sugar - tolerant yeast Zygosaccharomyces rouxii [ J ]. Food Bioteeh, 2005, 19 : 107 - 120.
  • 2RASHID M H, KATO F, MURATA A. Effect of microorganisms on the production of lipid and fatty acid composition of fermented fish meal [ J ]. Biosci Biotech Biochem, 1992,56 : 1058 - 1062.
  • 3YEN G C, LEE C A. Antioxidant activity of extracts from molds [J]. J Food Prot, 1996, 59:1327 -1330.
  • 4FREITAS de I, PINON N, BERDAGUE J L, et al. Kluyveromyces lactis but not Pichiafermentans used as adjunct culture modifies the olfactory profiles of Cantalet cheese [J]. J Dairy Sci, 2008, 91:531 -543.
  • 5KULLISAAR T, SONGISEPP E, MIKELSAAR M, et al. Antioxidative probiotic fermented goats ' milk decreases oxidative stress - mediated atherogenicity in human subjects [J]. Br J Nutr, 2003, 90:449-456.
  • 6LOWRY O H, ROSEBROUGH N J, LEWIS F A, et al. Protein measurements with the folin phenol reagent [J]. J Biol Chem, 1951, 193:265 -275.
  • 7LIN M Y, CHANG F J. Antioxidative effect of intestinal bacteria Bifidobacterium longum TCC 15708 and Lactobacillus acidophilus ATCC 4356 [ J ]. Dig Dis Sci, 2000, 45 : 1617 - 1622.
  • 8JEHRIG S C, ROHN S, KROH L W, et al. Antioxidative activity of ( 1 - 3 ), ( 1 - 6) - β - D - glucan from Saccharomyces cerevisiae grown on different media [J]. LWT, 2008, 41:868-877.
  • 9JEHRIG S C, ROHN S, KROH L W, et al. In vitro potential antioxidant activity of ( 1 - 3 ), ( 1 - 6) - D - Glucan and protein fractions from Saccharomyces cerevisiae cell walls [ J ]. J Agric Food Chem, 2007, 55:4710 - 4716.
  • 10NAYLIN N, SUGANUMA T, TAING O. Antioxidant activities of compounds isolated from fermented broth of Zygosaccharomyces rouxii [ J ]. Food Biotech, 2006, 20:131-141.

共引文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部