白藜芦醇对BGC-823细胞人程序化死亡分子5表达及凋亡的影响

Effects of resveratrol on expression of PDCD5 and apoptosis of BGC-823 cells

目的:探讨白藜芦醇对胃癌BGC-823细胞中凋亡相关基因人程序化死亡分子5(PDCD5)表达的影响,并观察白藜芦醇诱导BGC-823细胞凋亡的效应。方法:分别用25、50、100和200μmol/L的白藜芦醇处理BGC-823细胞24、48和72h后,采用免疫细胞化学和原位杂交方法检测PDCD5表达的变化;运用流式细胞仪和TUNEL法检测细胞凋亡情况。另设空白对照组和溶剂对照组。结果:同一时间点,6组PDCD5蛋白及mRNA的表达差异均有统计学意义(F蛋白=211.881、242.438和184.866,P均<0.001;FmRNA=207.366、148.411和158.661,P均<0.001);同一浓度,24、48和72h时点各组比较,PDCD5蛋白及mRNA的表达差异均无统计学意义(F蛋白=0.950、1.870、2.218和2.232,P均>0.05;FmRNA=1.267、2.376、1.221和2.292,P均>0.05)。各组BGC-823细胞凋亡指数比较,差异有统计学意义(F=230.069,P<0.001)。结论:凋亡相关基因PDCD5可能在白藜芦醇诱导胃癌BGC-823细胞凋亡过程中发挥重要作用。

Aim：To explore effects of resveratrol on expression of apoptosis-related gene PDCD5 and apoptosis in gastric cancer cell line BGC-823.Methods：BGC-823 was cultured and treated with different concentrations of resveratrol（25, 50, 100, and 200 μmol/L） for 24,48,and 72 h,respectively.The expressions of PDCD5 protein and mRNA were detected by immunocytochemistry and in situ hybridization, and cell apoptosis was analyzed by flow cytometry and TUNEL.Results：There were significant differences in PDCD5 protein and mRNA expression levels among the 6 groups at 24,48, and 72 h points（Fprotein=211.881,242.438,184.866, P0.001; FmRNA=207.366,148.411,158.661,P0.001）.In the same concentration（25,50,100,and 200 μmol/L） of resveratrol group, with the increasing of time,the expression levels of PDCD5 protein and mRNA had no significant difference（Fprotein=0.950,1.870,2.218,2.232,P0.05; FmRNA=1.267,2.376,1.221,2.292,P0.05）.BGC-823 cell apoptosis index had significant difference among the 6 groups（F=230.069,P0.001）.Conclusion： PDCD5 plays a key role in the gastric cancer cell apoptosis induced by resveratrol.