皮肤恶性黑色素瘤组织中Runx3与Smad4蛋白的表达

Expressions of Runx3 and Smad4 protein in cutaneous malignant melanoma tissue

目的:检测皮肤恶性黑色素瘤(CMM)组织中Runt相关转录因子3(Runx3)和Smad4蛋白的表达。方法:采用免疫组化SP法检测42例CMM、20例皮肤交界痣组织和20例正常皮肤组织中Runx3和Smad4蛋白的表达。结果:正常皮肤、皮肤交界痣及CMM组织中Runx3蛋白的阳性表达率分别为90.0%(18/20)、85.0%(17/20)和26.2%(11/42),3者相比,差异有统计学意义(χ2=31.371,P<0.001);正常皮肤、皮肤交界痣及CMM组织中Smad4蛋白的阳性表达率分别为85.0%(17/20)、85.0%(17/20)和42.9%(20/42),3者相比,差异有统计学意义(χ2=12.731,P=0.002)。CMM组织中Runx3和Smad4蛋白的表达存在关联性(rp=0.378,P=0.008)。结论:CMM组织中Runx3蛋白低表达或失表达,可能抑制了TGF-β/Smad4信号通路的激活,从而促进细胞的恶性转化和增殖。

Aim：To investigate the expressions of Runx3 and Smad4 protein in cutaneous malignant melanoma（CMM）.Methods：The expressions of Runx3 and Smad4 protein were detected in 42 cases of CMM,20 cases of junctional nevus and 20 cases of normal skin specimens by SP immunohistochemical technique.Results： The positive expression rate of Runx3 protein in normal skin specimens, junctional nevus and CMM tissue was 90.0%（18/20）,85.0%（17/20）,and 26.2%（11/42）,and there was significant difference（χ2=31.371,P0.001）.The positive expression rate of Smad4 protein in normal skin specimens, junctional nevus and CMM tissue was 85.0%（17/20）,85.0%（17/20）, and 42.9%（20/42）,and there was significant difference（χ2=12.731,P=0.002）.The expression of Runx3 was positively associated with that of Smad4 in CMM tissue（rp=0.378,P=0.008）.Conclusion：The low expression or deletion of Runx3 protein may effectively inhibit the over-activation of TGF-β/Smad4 signaling pathway, which might result in the malignant transformation and proliferation.