妊娠期二月桂酸二丁基锡暴露对子代雄性大鼠生殖系统的影响

Effects of Dibutyltin Dilaurat Exposure during Gestationperiod on Reproductive System of Male Offspring Rats

目的探讨妊娠期二月桂酸二丁基锡(DBTD)暴露对子代雄性大鼠性成熟后生殖系统的影响及其作用机制。方法将16只健康SPF级妊娠Wistar大鼠随机分为4组,分别为溶剂对照(玉米油)组和低(10 mg/kg)、中(20 mg/kg)、高剂量(30 mg/kg)DBTD染毒组,每组4只。采用灌胃方式进行染毒,染毒容量为5 ml/kg,自妊娠第12～20天连续染毒。仔鼠出生后第70天,每组随机抽取10只雄性大鼠,称重后处死,测定睾丸和附睾重量及其脏器系数、附睾精子数以及血清中黄体生成素(LH)、卵泡刺激素(FSH)和睾酮(T)以及睾丸组织中睾酮(T)的水平,并观察睾丸组织病理学改变。结果各剂量DBTD染毒组子代雄性大鼠体重、附睾重量及其脏器系数、血清中LH和FSH水平与溶剂对照组相比,差异均无统计学意义。与溶剂对照组比较,高剂量DBTD染毒组子代雄性大鼠睾丸重量及其脏器系数,中、高剂量DBTD染毒组子代雄性大鼠附睾精子数较高,差异均有统计学意义(P<0.05或P<0.01)。且随着DBTD染毒剂量的升高,子代雄性大鼠睾丸重量及其脏器系数以及附睾精子数均呈升高趋势。与溶剂对照组比较,高剂量DBTD染毒组子代雄性大鼠血清T水平和各剂量DBTD染毒组子代雄性大鼠睾丸T水平均较高,差异有统计学意义(P<0.05或P<0.01)。结论在本实验染毒时间和剂量范围内,孕期DBTD染毒可干扰子代雄性大鼠体内T的合成和代谢,从而促进睾丸发育和精子形成。

Objective To explore the effects of dibutyltin dilaurate（DBTD） exposure during gestationperiod on reproductive system in the mature male offspring of Wistar rats and its mechanism.Methods The specific pathogen free（SPF） pregnant rats were randomly divided into four groups,four in each,including the control（corn oil）,the low dosage（10 mg/kg）,the moderate dosage（20 mg/kg） and the high dosage（30 mg/kg）.The animals were treated with DBTD by gavage from 12th to 20th days of gestation and the volume of DBTD solution administered was adjusted to 5 ml/kg of body weight.At the 70th postnatal day,10 male mature rats were selected randomly from each group for the measurement of the weights of testis and epididymis,the viscera coefficient of testis and epididymis,sperm count in epididymis,the hormones including luteinizing hormone（LH）,follicle stimulating hormone（FSH）,testosterone（T） in serum and testosterone in testis,and then observed pathologic changes in testis.Results There were no significant differences in body weight,epididymis weight,the viscera coefficient of epididymis,LH and FSH in serum between the exposure groups and the control（P0.05）.The tesis weight and viscera coefficient of the testis in the high dosage and the sperm counts in the moderate dosage and the high dosage increased significantly compared with the control（P0.05 or P0.01）.The tesis weight,viscera coefficient of testis and the sperm counts increased with the increasing dosage.T in serum in high dosage and T in testis in each treated group increased significantly compared with the control（P0.05 or P0.01）.Conclusion In the experimental condition of the present study,DBTD exposure during gestationperiod shows a promotive effect on the development of testis and the spermatogenesis in offspring rats by interfering synthesis and metabolism of T.