亚砷酸钠染毒对大鼠皮肤及其他脏器损伤的实验研究

Study on Skin and Organs Toxicity in Rats Induced by Sodium Arsenite

目的研究不同剂量的亚砷酸钠(NaAsO2)对大鼠皮肤和脏器的毒性损伤。方法将40只SPF级SD大鼠按性别和体重随机分成对照组、低剂量组、中剂量组和高剂量组,分别自由饮用蒸馏水、12.5、25和50 mg/L的NaAsO2水溶液,连续染毒90 d后取材,采用火焰原子吸收法测定大鼠皮肤和心、肝、脑、肾主要脏器中的砷含量,HE染色后光镜下观察大鼠皮肤表皮结构及心、肝、肾、脑脏器的组织学变化。结果与对照组比较,砷染毒组大鼠均有不同程度的体重减轻、脏器系数升高、体内各组织中砷蓄积量增高的亚慢性砷中毒表现,差异有统计学意义(P<0.05);不同剂量的NaAsO2染毒组大鼠皮肤及心、肝、脑、肾组织出现不同程度的病理损伤。结论 NaAsO2对大鼠皮肤及心、肝、脑、肾各脏器存在明显的损伤作用。

Objective To investigate the toxicity of different doses of sodium arsenic（NaAsO2） to rats skin and organs.Methods Forty SD rats were randomly divided into four groups,10 in each,including control group（C）,low dose arsenic poisoning group（L）,moderate dose arsenic poisoning group（M）,and high dose arsenic poisoning group（H）.Rats of control group were fed with distilled water.Rats in group L,group M and group H were fed with water contained sodium arsenic at the concentrations of 12.5 mg/L,25 mg/L and 50 mg/L respectively.After 90 days of treatment,the arsenic content in skin and organs of rats was determined with atomic absorption spectrophotometer;The epidermal structure of rat skin and the histological changes of heart,liver,kidney,brain organs were observed by light microscope after HE staining.Results The body weight decreased and the organ coefficient increased in each dose group.The arsenic in skin,heart,liver,brain and kidney significantly increased as the dose of NaAsO2 increased（P0.05）.The different doses of sodium arsenite can injury the normal structure of the skin and organs of rats.Conclusion NaAsO2 can cause obvious injury in the skin and organs of rats.