胰腺癌淋巴结中血管内皮生长因子-C及其受体-3蛋白表达及其临床意义

Expression of vascular endothelial growth factor-C and vascular endothelial growth factor receptor-3 protein in nymph nodes of pancreatic cancer and its clinical significance

目的通过胰腺癌标本组织和淋巴结中血管内皮生长因子(VEGF)-C及其受体(VEGFR 3)蛋白的检测,探讨VEGF-C及VEGFR-3蛋白表达与胰腺癌淋巴结转移的相关性。方法采用免疫组织化学方法检测胰腺癌组织标本及其淋巴结中VEGF-C及VEGFR-3蛋白的表达,分析VEGF-C及VEGFR-3蛋白表达与胰腺癌的临床病理特征和淋巴结转移的相关性。结果胰腺癌组织标本中VEGF-C、VEGFR-3蛋白表达的阳性率分别为80%(20/25)、92%(23/25)。一般情况下,VEGF-C、VEGFR-3蛋白在胰腺癌组织周边的表达强于癌灶中央。VEGF-C、VEGFR-3蛋白在常规病理学检测(苏木精-伊红染色)阳性的淋巴结内的表达明显强于阴性淋巴结中(P值均<0.05)。不同分化程度(x^2=4.17,P=0.041)、有无十二指肠侵犯(x^2=5.77,P=0.018)的胰腺癌标本中VEGF-C表达的差异均有统计学意义;不同分化程度(x^2=1.45,P=0.221)、有无十二指肠侵犯(x^2=2.01,P=0.167)的胰腺癌标本中VEGFR-3蛋白表达的差异均无统计学意义。结论 VEGF C和VEGFR-3蛋白的表达与胰腺癌淋巴结转移密切相关,推测其与诱导胰腺癌淋巴管生成,促进肿瘤细胞淋巴道转移有关。

Objective To observe the expression of vascular endothelial growth factor-C（VEGF-C） and vascular endothelial growth factor receptor-3 protein（VEGFR-3） in pancreatic cancer tissues and lymph nodes,so as to discuss the relationship between VEGF-C and VEGFR-3 with the lymphatic metastasis of pancreatic cancer. Methods The expression of VEGF-C and VEGFR-3 protein in pancreatic cancer tissues and lymph nodes was detected immunohistochemically in 25 patients who had undergone pancreaticoduodenectomy with extended lymphadenectomy.The relation between the expression of VEGF-C and VEGFR-3 protein and lymphatic metastasis of pancreatic cancer was also analyzed.Results The positive rates of VEGF-C and VEGFR-3 in pancreatic cancer specimens were 80%（20/25）,92%（23/25）,respectively.The expression of VEGF-C and VEGFR-3 at the border of the tumor specimen was significantly higher than that at the center of the tumor;VEGF-C and VEGFR- 3 protein expression was stronger in patients with a positive result in HE staining compared with those with negative results（P0.05）.The expression of VEGF-C was significantly different between patients with different differentiation degree（x^2=4.17,P = 0.041） and the presence of duodenal infiltration（x^2=5.77,P = 0.018）. The expression of VEGFR-3 was not significantly different in patients with different differential diagnoses. Conclusion The expression of VEGF-C and VEGFR-3 protein is closely related to the incidence of lymph node metastasis of the pancreatic head carcinoma.VEGF-C and VEGFR-3 may induce lymphangiogenisis in pancreatic cancer,and thus promote the tumor cell lymph metastasis.