摘要
Objective To investigate expression and localization of Calbindin-d28k (CaBP-d28k) in human ovary. Methods The expression of the CaBP-d28k protein and gene in 23 human ovarian samples was measured by immunohistochemistry and reverse transcription polymerase chain reaction (RTPCR) methods. Results Positive expression of the CaBP-d28k protein and mRNA was observed in human ovary. The ovarian expression of the CaBP-d28k protein appeared in the cytoplasm of oocytes, granulose cells, theca cells, and two types of cells of corpus luteum. From primordial follicle, primary follicle to secondary follicle, increased CaBP- d28k levels were showed (P〈0. 05), and the CaBP-d28k expression in secondary follicle had the highest level. Conclusion Human ovarian tissue locally expresses CaBP-d28k, and its expression level increases with follicular development.
Objective To investigate expression and localization of Calbindin-d28k (CaBP-d28k) in human ovary. Methods The expression of the CaBP-d28k protein and gene in 23 human ovarian samples was measured by immunohistochemistry and reverse transcription polymerase chain reaction (RTPCR) methods. Results Positive expression of the CaBP-d28k protein and mRNA was observed in human ovary. The ovarian expression of the CaBP-d28k protein appeared in the cytoplasm of oocytes, granulose cells, theca cells, and two types of cells of corpus luteum. From primordial follicle, primary follicle to secondary follicle, increased CaBP- d28k levels were showed (P〈0. 05), and the CaBP-d28k expression in secondary follicle had the highest level. Conclusion Human ovarian tissue locally expresses CaBP-d28k, and its expression level increases with follicular development.
