川乌药酒中毒冠心病大鼠模型的制备

Estabishment of Medicine Wine Poisioning Rat Mode with Coronary Heart Disease

目的:探讨建立川乌药酒中毒冠心病大鼠模型的方法。方法:将40只6月龄Wistar大鼠,随机分为A、B两组,A组经胸骨正中线入路建立缺血模型;B组经肋间入路建立缺血模型。2周后将成功建立的模型随机分为低、中、高剂量三组,分别以不同剂量的自制川乌药酒灌胃,利用高效液相色谱技术测定中毒大鼠血液中的乌头碱含量。结果:B组2周的成活率明显高于A组(P<0.05)。药酒中乌头碱成分吸收迅速,在灌胃后血液即可检测到乌头碱成分,低、中、高三组间有显著性差异(P<0.05和P<0.01),且随着时间的推移,毒性成分吸收增加。结论:B组的手术入路建立冠心病大鼠模型成功率高。以低剂量的自制药酒灌胃是建立川乌药酒中毒冠心病大鼠模型的理想剂量。

Objective：To explore the method of establishment of medicine wine poisioning rat mode with coronary heart disease. Medotbs： 40 Wistar rats of 6 months were randomly divided two groups（A and B）, and the two groups were built mode with coronary heart disease through different operation ways. After two weeks, rat modes were randomly divided three groups again, and were accepted medicine wine at the different dosages. Then aconitine contents were determined by high performance liquid chromatography.Results：The survival rate of B group was obviously higher. Aconitine of medicine wine was absorbed quickly after reception of medicine wine. With the development of time, aconitine was more and more absorbed. Content of aconitine in the three groups had significantly different.Conclusion：Operention method of B group could obviously improve survival rate about estabishment of rat mode with coronary heart disease. Lower dose medicine wine is optimal dose for estabishment of medicine wine poisioning rat mode with coronary heart disease.