高原兔肝脏火器伤后GSH、SOD和MDA变化特点及意义

Dynamic changes of GSH,SOD,MDA after firearm wound in livers of rabbits in high altitude

目的:探讨在高原环境下兔肝脏火器伤后全血谷胱甘肽(GSH)含量、血清超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量的动态变化规律。方法:144只新西兰大白兔随机分为平原肝脏火器伤组(A组)、初入高原肝脏火器伤组(B组)、适应高原肝脏火器伤组(C组),各组又细分为伤后0.5、2、6、12、24、48、72h和正常对照组,每组6只。在不同海拔环境下成功建立肝脏火器伤模型后,分别于各时间点采集血液标本,检测全血GSH含量、血清SOD活力和MDA含量。结果:①兔肝脏火器伤后与正常组比较,伤后GSH含量、SOD活力早期略有升高,以后逐渐下降,到达低谷后逐渐恢复。而MDA在伤后持续升高,12h达峰值(P<0.01),继而进行性下降,72h仍高于正常。②初入高原组与平原组比较,GSH含量、SOD活力早期升高及以后到达低谷和MDA到达峰值水平(P<0.01),初入高原组更显著。③适应高原组与平原组比较,GSH含量、SOD活力降低幅度(P<0.05)和MDA升高程度,适应高原组更显著。结论:肝脏火器伤破坏了体内氧化与抗氧化之间的平衡,尤其在高原缺氧环境下更为明显,提示氧自由基可能参与肝脏火器伤后机体的病理生理过程。

Objective： To investigate the dynamic changes of GSH contect in whole blood,SOD activity and MDA content in serum after firearm wound in livers of rabbits in high altitude.Methods： One hundred and forty-four New Zealand rabbits were divided randomly into： normal environment group（groupA）,newly entering high altitude group（groupB）,adaptation to high altitude group（groupC）,and each group were subdivided into 0.5,2,6,12,24,48,72h after firearm wound and control group,n=6.After successfully eatablished firearm wound model of livers in different attitudes,blood samples were collected respectively at diffierent time points,detecting GSH contect in whole blood,SOD activity and MDA content in serum.Results： ①compared with control group,GSH contect and SOD activity slightly rose early after injury,and gradually declined until reached the minimun,then progressively rose.And MDA content rose gradually after injury,and reached the peak at 12h,and declined gradually.②compared with normal environment group,newly entering high altitude group of the extent of GSH contect,SOD activity rose early and then reached the low point,and MDA content reached the peak is more salient.③compared with normal environment group,adaptation to high altitude group of the extent of GSH contect,SOD activity declined and MDA content rose is more salient.Conclusion： Firearm wound in the liver breaks the balance of peroxidation and anti-oxidation system,especially more obvious in the high altitude environment,which reveals that oxygen free radicals may be involved in the body pathophysiology process after firearm wound in the liver.