HIF-1α、HSP90α在肝细胞癌中的表达及临床意义

Expression and Clinical Signficance of HIF-1α and HSP90α in Hepatocellular Carcinoma

目的:探讨缺氧诱导因子-1α(HIF-1α)和热休克蛋白9Oα(HSP90α)在肝细胞癌中的表达及其与临床病理特征之间的关系。方法:采用免疫组织化学Envision二步法检测H HIF-1α和HSP90α蛋白在65例肝细胞癌和癌旁组织、20例正常肝组织中的表达,并分析其与肝细胞癌临床病理因素的关系。结果:HCC组织中HIF-1α和HSP90α阳性表达率均明显高于癌旁组织及正常肝脏组织,差异均具有统计学意义(P<0.01)。HIF-1α在HCC组织中的表达与肿瘤大小、临床分期、病理分级、有无淋巴结转移、有无门静脉癌栓密切相关(P<0.01),HSP90α在HCC组织中的表达与临床分期、病理分级、有无淋巴结转移、有无门静脉癌栓密切相关(P<0.01)。Spearman相关性检验分析提示HIF-1α和HSP90α表达阳性程度呈正相关(r=0.536,P<0.01)。结论:HIF-1α和HSP90α参与肝细胞癌的发生、发展,并起协同作用,可能作为判断肝细胞癌预后的指标。

Objective： To investigate the expression of HIF-1αand HSP90α in hepatocellular carcinoma（HCC） and to analyze the relationships between protein expression and clinicopathologic factors.Methods： The expression of HIF-1α and HSP90αprotein was detected with immunohistochemical Elivision TM plus non-hiotin technique in 65 cases of HCC and pericarcinoma tissues,20 cases of normal tissues.Results： The positive expression rates of HIF-1αand HSP90αprotein in HCC were significantly higher than that in pericarcinoma tissues and normal tissues respectively（P0.01）.The expression of HIF-1αwere positively related to tumor size,tumor clinica1 stage,differentiation degree,lymph node metascasis and portal vein cancer-embolus（P0.01）.The expression of HSP90αwere positively related to tumor clinica1 stage,differentiation degree,lymph node metascasis and portal vein cancer-embolus（P0.01）.The ex-pression of HIF-1αwas positiye1y correlated with HSP90α（r=0.536,P0.01）.Conclusion： Expression of HIF-1αand HSP90αprotein may play important roles in the carcinogenesis and development of HCC.The detection of HIF-1αand HSP90α expression may serve as one of the important parameters in evaluating the prognosis of HCC.