HCV-RNA和抗-HCV的联合检测在丙型肝炎确诊中的临床意义

Clinical significance of joint detection of HCV-RNA and anti-HCV in diagnosis of hepatitis C

目的:探讨抗-HCV和HCV-RNA的联合检测在丙型肝炎确诊中的临床意义。方法:采用ELISA法检测抗-HCV及实时荧光定量PCR检测HCV-RNA。结果:在急性丙型肝炎组中抗-HCV和HCV-RNA同时阳性所占百分率(29.8%)显著低于慢性丙型肝炎患者(62.3%)(P<0.05);抗-HCV阴性和HCV-RNA阳性在急性丙型肝炎组所占百分率(58.3%)显著高于慢性丙型肝炎患者(24.6%)(P<0.05);经相关性分析,ALT含量与HCV-RNA含量呈正相关性(r=0.725,P<0.05)。结论:临床抗-HCV和HCV-RNA的联合检测有助于丙型肝炎的早期明确诊断。

Objective： To analyze the clinical significance of joint detection of HCV-RNA and anti-HCV in diagnosis of hepatitis C.Methods： We detected anti-HCV by enzyme linked immunosorbent assay and HCV-RNA by real-time fluorescent quantitation PCR.Results： The rate of positive reaction（29.8%） to anti-HCV and HCV-RNA in acute hepatitis C group was lower than that（62.3%） in chronic hepatitis C group（P 0.05）;The rate（58.3%）of positive in HCV-RNA and negtive in anti-HCV in acute hepatitis C group was higher than that（24.6%）in chronic hepatitis C group（P 0.05）.The correlation analysis showed that HCV-RNA and ALT levels were positively correlated（r =0.725,P0.05）.Conclusions： Clinical joint detection of HCV-RNA and anti-HCV contributes to the early diagnosis of hepatitis C.