毛细管电泳法测定苷肽胶囊中3种核苷酸

Capillary Electrophoretic Determination of 3 Nucleotides in G-P Health-care Capsule

提出了毛细管电泳法同时分离测定苷肽胶囊中3种核苷酸腺嘌呤-5′-单磷酸、胞嘧啶-5′-单磷酸和鸟嘌呤-5′-单磷酸含量的方法。以15 mmol.L-1硼砂+35 mmol.L-1碳酸钠+60 mmol.L-1羟丙基-β-环糊精混合溶液为电解质溶液,运行电压30 kV,于254 nm波长处进行紫外检测。3种核苷酸的线性范围依次为5.0～80.0,8.0～80.0,8.0～80.0 mg.L-1,检出限(3S/N)依次为2.0,3.0,3.0 mg.L-1。采用该方法对苷肽胶囊样品中3种核苷酸的含量分别进行了测定,所得加标回收率在92.1%～102.7%之间,相对标准偏差(n=5)均小于等于5.0%。

A method for the simultaneous separation and determination of 3 nucleotides, i. e. adenosine 5＇- monophosphate （AMP）, cytidine 5＇-monophosphate （CMP） and guanosine 5＇-monophosphate （GMP）, in the health-care capsule containing glucoside and peptide （abbreviated as G-P capsule） by capillary electropboresis was proposed. A mixture of solutions of 15 mmol . L-1 sodium borate, 35 mmol. L-1 sodium carbonate and 60 mmol . L-1 hydroxypropyl-β-cyclodextrin was used as background electrolyte, and moving voltage of 30 kV was applied. UV-detection at the wavelength of 254 nm was adopted. Linearity ranges between 5.0--80. 0 mg . L-1 （for AMP） and 8.0--80.0 mg . L-1 （for CMP and GMP） were obtained with detection limits （3S/N） of 2. 0, 3. 0 and 3. 0 mg.L-1 respectively. Recovery and precision of the method were tested by standard addition method, the results of recovery found were in the range of 92. 1%- 102. 7 %and RSD＇s （n= 5） were less than or equal to 5. 0%.