摘要
采用反相高效液相色谱法对血清中阿立哌唑及其代谢物脱氢阿立哌唑进行测定。血清样品经乙醚提取,所得提取液再经35℃氮气吹干,残留物经流动相溶解后,用Zorbax-C18色谱柱分离,用紫外检测器于254 nm波长处测定。阿立哌唑及其代谢物脱氢阿立哌唑的线性范围分别为4.0～2 000μg.L-1和4.0～1 500μg.L-1;测定下限(10S/N)均为4.0μg.L-1。相对回收率分别为100.3%～102.7%和93.7%～103.9%;日内和日间相对标准偏差(n=5)均小于7%。
A method of RP-HPLC for determination of aripiprazole and its metabolite,dehydro-aripiprazole was proposed.Sample of blood serum was extracted with ethyl ether and the eluate obtained was evaporated to dryness by N2-blowing at 35 ℃.The residue was dissolved with the mobile phase mixture and separated by passing through Zorbax-C18 column,and the eluate from the column was used for HPLC analysis with UV detector at the wavelength of 254 nm.Linearity ranges of aripiprazole and dehydro-aripiprazole were kept from 4.0 to 2 000 μg·L-1 and 4.0 to 1 500 μg·L-1 respectively,with same lower limits of determination（10S/N） of 4.0 μg·L-1.Values of relative recovery of aripiprazole and dehydro-aripiprazole in the range of 100.3%-102.7% and 93.7%-103.9% were found respectively.Values of intraday and interday relative standard deviation（n=5） were all less than 7%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2010年第11期1293-1295,1299,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
国家重点基础研究发展计划(2007CB512306)
