基质固相分散-离子色谱法测定烟草中糖和多元醇

Ion-chromatographic Determination of Saccharides and Polyols in Tobacco with Separation by Matrix Solid Phase Dispersion

采用基质固相分散法提取净化烟草中的糖和多元醇,并用离子色谱法进行测定。将烟草试样与石墨化炭黑球(质量比为1比4)充分研磨后装柱,用热水洗脱,洗脱液中的糖和多元醇用离子色谱测定。以CarboPac MA1阴离子色谱柱(4 mm×250 mm,5μm)为固定相,用0.5 mol.L-1氢氧化钠溶液为流动相分离,用脉冲安培检测器检测。烟草试样中甘油、丙二醇、木糖醇、山梨醇、葡萄糖、果糖、蔗糖、麦芽糖和半乳糖9种成分在50 min内完全分离。方法的加标回收率在94.6%～105.3%之间,相对标准偏差(n=7)均小于3.0%。方法的检出限均小于50μg.L-1。

Saccharides and polyols in tobacco sample were separated by the method of matrix solid phase dispersion and determined by ion chromatography（IC）.The sample was mixed with graphitized carbon black granules in the mass ratio of 1 to 4 and ground in a motar to make it a homogeneous dispersion.The dispersion was then filled into a solid phase extraction column.The SPE column was eluted with hot water to dissolve the saccharides and polyols from the sample in the dispersion.CarboPac MA1（4 mm×250 mm,5 μm） anion exchange column was used as stationary phase,and 0.5 mol·L-1 sodium hydroxide solution was used as mobile phase.Conductivity detection was adopted.Nine components in tobacco,including glycero1,propylene glyco1,xylito1,sorbito1,fructose,glucose,sucrose,maltose and galactito1 were completely separated within 50 min.Values of detection limits（3S/N） found were all below 50 μg·L-1.Tests for recovery and precision were made,giving values of recovery ranged from 94.6%-105.3%,and of RSD′s（n=7） less than 3.0%.