注射用葛根素在小鼠体内的药动学研究

Study on pharmacokinetics of puerarin for injected in mice

目的研究注射用葛根素在小鼠体内的药动学规律。方法小鼠尾静脉注射葛根素80mg.kg-1后,采用乙晴沉淀蛋白,高效液相色谱法测定血浆中葛根素的含量。结果采用3p97处理数据,葛根素的血药浓度-时间曲线符合二室开放模型(权重1/C2),其主要药动学参数分别为k=0.794,t1/2=0.87h,血药浓度-时间曲线下面积(AUC)=64.88(mg.L-1).h,消除率〔CL(S)〕=1.23 mg.kg-1/h/(mg.L)。结论明确了葛根素在小鼠体内的药动学特征。

OBJECTIVE To observe the pharmacokinetic characteristics of Puerarin injection in rabbits.METHODS After giving of injection of Puerarin i.v.80mg·kg-1 into mice,HPLC was used to determine the puerarin levels in the biological samples.RESULTS The plasma concentration-time course of puerarin fitted well to two-compartment model and with the following pharmacokinetic parameters：k=0.794,（t1/2）=0.87 h,（AUC）=64.88（mg·L-1）·h,CL（S）=1.23 mg（kg·h）/（mg·L）.CONCLUSION The pharmacokinetic characteristics of puerarin in mice was understood.