摘要
目的建立测定地黄丸中马钱苷含量的高效液相色谱法。方法采用高效液相色谱法。以Hypersil ODS C18(250mm×4.6mm,5μm)为色谱柱;流动相为四氢呋喃-甲醇-乙腈-0.05%磷酸(1:4:8:87);检测波长:236nm;流速:1.0mL.min-1。结果加样回收率平均为97.08%,RSD为0.68%(n=6)。结论试验结果表明,该方法准确、灵敏度高,重复性好。
OBJECTIVE To establish a high performance liquid hromatography（HPLC） method for determination of loganin in dihuangwan.METHODS HPLC method was carried on C18 column,using Tetrahydrofuran Methanol Acetonitrile-0.05% Phksphoric Acid water（1∶4∶8∶87） as mobile phase,detector temperature was set at 40℃,detection wavelength was set at 236 nm.RESULTS The mean recovery of loganin in luiweiwan was 97.08,RSD =0.68%（n=6）.CONCLUSION The results showed that this method is reliable and accurate.
出处
《海峡药学》
2010年第12期67-69,共3页
Strait Pharmaceutical Journal
