HPLC法同时测定苯巴比妥、苯妥英、卡马西平和茶碱血药浓度

Simultaneous Determination of Theophylline,Phenobarbital,Phenytoin and Carbamazepine in Human Serum by HPLC

目的建立同时测定血清中茶碱(TPH)、苯巴比妥(PBB)、苯妥英(PHT)和卡马西平(CBZ)浓度的HPLC法。方法取患者血清,经二氯甲烷提取后岛津LC-20A Prodigy ODS C18柱(4.6mm×250mm,5μm)上分析,流动相为甲醇-水(48:52),流速为1.0mL.min-1,检测波长为254nm,柱温40℃。结果 TPH,PB,PT和CBZ的线性范围和相关系数分别为0.26～80.88(0.9994),1.0～80.56(0.9992),1.02～81.6(0.9990),0.27～40.22(0.9996)μg.mL-1,最低检测1浓度分别为0.09,0.20,0.24,0.03μg.mL-1,方法回收率分别为99.0%,99.4%,100.3%,99.6%日内、日间RSD均<3.0%,绝对回收率分别为91.9%,95.4%,92.3%,95.9%,RSD均<2%。结论该方法简便、灵敏、实用、有良好的分离效果,适用于治疗药物监测及药物动力学实验。

OBJECTIVE To establish an external standard HPLC method for the simultaneous determination of theophylline（TPH）,phenobarbital（PBB）,phenytoin（PHT）,carbamazepine（CBZ）and in human serum.METHODS SHIMADZU LC-20A Prodigy ODS C18 column（4.6mm×250mm,5μm）was used.The mobile phase was methanol-water（48∶52）.The flow rate was 1.0mL·min-1 and the detection wavelength was 254 nm.The column temperature was 40℃.RESULTS The good linearity of TPH,PBB,PHT and CBZ were obtained over the range of 0.26～80.88,1.0～80.56,1.02～81.6,0.27～40.22μg·mL-1 with correlation coefficient r=0.9994,0.9992,0.9990,0.9996,respectively.The limit concentration of detection of TPH,PBB,PHT and CBZ were 0.09,0.20,0.24 and 0.03μg·mL-1,respectively.The average recovery of TPH,PBB,PHT and CBZ were 99.0%,99.4%,100.3%,99.6%,respectively.The RSD for the inter-and intra-day of four drugs were less than 3.0%.Absolute recovery were 91.9%,95.4%,92.3% and 95.9%,respectively.The RSD were less than 2%.CONCLUSION The method is convenient,sensitive,practical and has well separating effects.It is suitable for therapeutic drug monitoring and pharmacodynamics experiment of TPH,PBB,PHT and CBZ.