膀胱癌患者外周血髓系来源的抑制细胞的比例及其临床意义

The prevalence of myeloid-derived suppressor cells in the peripheral blood of patients with bladder cancer

目的分析髓系来源的抑制细胞(myeloid-derived suppressor cells,MDSC)在膀胱癌患者外周血中的分布,初步探讨其临床意义。方法采用流式细胞术检测62例膀胱癌患者及20例健康人外周血中MDSC的比例,分析MDSC比例与临床病理特征和CD4+CD25high调节性T细胞(Treg)的关系。结果与正常对照(0.122±0.043)%相比,膀胱癌患者外周血中MDSC比例明显增加(0.679±0.438)%,二者差异有显著性(P<0.01);手术后,患者外周血中MDSC的比例明显下降,与术前比较有统计学意义(P<0.05);MDSC水平与膀胱癌肿瘤分期明显相关,而与肿瘤的分化程度和患者外周血Treg水平无显著相关(P>0.05)。结论膀胱癌患者外周血MDSC细胞水平明显升高,可能与肿瘤免疫功能低下及肿瘤发生发展密切相关。

Objective To investigate the prevalence of myeloid-derived suppressor cells（MDSCs）in the peripheral blood of patients with bladder carcinoma.Methods Flow cytometry was used to evaluate the prevalence of MDSCs in the peripheral blood of 62 patients with bladder carcinoma and 20 healthy people.The change of the frequency of MDSCs in the peripheral blood of 10 patients before and after operation was investigated.Furthermore,the relationship of peripheral blood MDSC level with pathological characteristics and the prevalence of CD4＋CD25high regulatery T cells（Tregs） was determined statistically.Results Compared with normal control〔（0.122±0.043）%〕,the prevalence of MDSC in the peripheral blood of patients with bladder carcinoma was highly increased〔（0.679±0.438）%（P0.01）〕.After the operation,the frequency of MDSCs was decreased（P0.05）.Circulating MDSCs was significantly increased in cancer patients of all stages compared with healthy volunteers.A significant correlation between circulating MDSCs and clinical tumor stage was also observed.However,no significant correlation between circulating MDSCs and the differentiation state of cancer,the prevalence of Tregs was observed.Conclusion The prevalence of MDSCs is increased in the peripheral blood of patients with bladder cancer.These cells may negatively regulate antitumor immune response of patients.