自体来源树突状细胞和细胞因子诱导的杀伤细胞混合培养对原代乳腺癌细胞的杀伤作用

Study of primary cells of breast cancer with special immune response induced by CIK co-cultured with serf-dendritic cells

目的探讨原代乳腺癌细胞冻融抗原负荷自体来源树突状细胞（DC）与细胞因子诱导的杀伤细胞（CIK）细胞混合培养后对原代乳腺癌细胞杀伤活性的影响。方法取对数生长期的原代乳腺癌细胞制备肿瘤抗原（Ag），用自体外周血单个核细胞（PBMC）分别制备DC和CIK细胞；将负荷Ag的DC和CIK细胞共培养，诱导Ag-DC-CIK细胞。采用流式细胞术检测DC免疫表型，MTT法检测分离的PBMC、CIK、Ag-CIK和Ag．DC-CIK对原代乳腺癌细胞的杀伤活性。结果PBMC、CIK、Ag-CIK和Ag-DC-CIK对原代乳腺癌肿瘤细胞杀伤活性分别是（34．35±3．28）％、（45．91±2．78）％、（50．88±3．22）％、（62．10±5．94）％，实验组细胞（Ag-DC-CIK）对原代乳腺癌细胞的杀伤能力为（62．10±5．94）％，明显高于对照组（P〈0．01）。结论肿瘤抗原负荷的DC能增强CIK细胞对靶细胞的杀伤活性，为DC的免疫治疗提供了新的思路。

Objective To investigate effect to kill primary breast cancer cells by dendritic cell （DC） with self-freeze thawing antigens of primary breast cancer cells co-cultured with eytokine induced killer cell （CIK）. Methods Self-neoplasm antigen by primary cells of breast cancer in log phase growth was prepared and DC and CIK cells was cultured from peripheral blood. The CIK of co-cultured DC loaded self-neoplasm antigen was compared to PBMC, CIK cells with self-neoplasm antigen and the single CIK cells. Results Killing efficiency of PBMC, CIK, Ag-CIK and Ag-DC-CIK were （34.35±3.28） %, （45.91±2.78） %, （50.88± 3.22） %, （62.10±5.94） %. There were significant difference between Ag-DC-CIK group and CIK-Ag and CIK group to primary cells of breast cancer （P 〈0.01）. Conclusion The CIK induced from cocuhured DC loaded with self-neoplasm antigen show a special killing activity to self-primary tumor cells.