摘要
目的探讨原代乳腺癌细胞冻融抗原负荷自体来源树突状细胞(DC)与细胞因子诱导的杀伤细胞(CIK)细胞混合培养后对原代乳腺癌细胞杀伤活性的影响。方法取对数生长期的原代乳腺癌细胞制备肿瘤抗原(Ag),用自体外周血单个核细胞(PBMC)分别制备DC和CIK细胞;将负荷Ag的DC和CIK细胞共培养,诱导Ag-DC-CIK细胞。采用流式细胞术检测DC免疫表型,MTT法检测分离的PBMC、CIK、Ag-CIK和Ag.DC-CIK对原代乳腺癌细胞的杀伤活性。结果PBMC、CIK、Ag-CIK和Ag-DC-CIK对原代乳腺癌肿瘤细胞杀伤活性分别是(34.35±3.28)%、(45.91±2.78)%、(50.88±3.22)%、(62.10±5.94)%,实验组细胞(Ag-DC-CIK)对原代乳腺癌细胞的杀伤能力为(62.10±5.94)%,明显高于对照组(P〈0.01)。结论肿瘤抗原负荷的DC能增强CIK细胞对靶细胞的杀伤活性,为DC的免疫治疗提供了新的思路。
Objective To investigate effect to kill primary breast cancer cells by dendritic cell (DC) with self-freeze thawing antigens of primary breast cancer cells co-cultured with eytokine induced killer cell (CIK). Methods Self-neoplasm antigen by primary cells of breast cancer in log phase growth was prepared and DC and CIK cells was cultured from peripheral blood. The CIK of co-cultured DC loaded self-neoplasm antigen was compared to PBMC, CIK cells with self-neoplasm antigen and the single CIK cells. Results Killing efficiency of PBMC, CIK, Ag-CIK and Ag-DC-CIK were (34.35±3.28) %, (45.91±2.78) %, (50.88± 3.22) %, (62.10±5.94) %. There were significant difference between Ag-DC-CIK group and CIK-Ag and CIK group to primary cells of breast cancer (P 〈0.01). Conclusion The CIK induced from cocuhured DC loaded with self-neoplasm antigen show a special killing activity to self-primary tumor cells.
出处
《肿瘤研究与临床》
CAS
2010年第12期814-816,共3页
Cancer Research and Clinic