三磷酸腺苷生物荧光体外药物敏感检测法在贲门癌化疗中的应用

Application of ATP-TCA in chemotherapy of human cardia cancer

目的采用三磷酸腺苷生物荧光体外药物敏感检测法（ATP-TCA）对贲门癌手术标本进行体外药物敏感检测，为贲门癌的临床化疗选择提供依据。方法用ATP-TCA法检测42例贲门癌手术标本对8种常用化疗药物的敏感度并进行评价，分析临床分型与敏感度的相关性。结果不同标本的敏感度有明显的不同，敏感度由高至低为多柔比星（ADM）〉丝裂霉素（MMC）〉紫杉醇（PTX）〉5-氟尿嘧啶（5-Fu）〉羟基喜树碱（HCT）=依托泊苷（vP16）〉顺铂（DDP）〉异环磷酰胺（IFO），敏感度依次为50．00％、35．45％、31．25％、23．53％、10．53％、10．53％、7．69％、5．00％，同一药物在不同病理分期的敏感度差异无统计学意义。结论ATP-TCA可为啬门痛的临床个体化化疔提供萤要的指导作用．

Objective To investigate the drug sensitivity of cardia tumors in vitro by ATP-TCA, and to provide basis of clinic chemotherapy. Methods 42 eardia tumors were tested by ATP-TCA to determine and assess the drug sensitivity of 8 common ehemotherapeutics. Correlation analysis was done between the clinical classification and the drug sensitivity. Results The drug sensitivity had significant difference among cardia tumors. From high to low, it was ADM〉MMC〉PTX〉 5-Fu〉HCT ：VP16〉CDDP〉IFO, Sensitive degree were 50.00 %, 35.45 %, 31.25 %, 23.53 %, 10.53 %, 10.53 %, 7.69 %, 5 %. Sensitivity of the same drug in different pathologic stages had no significant difference. Conclusion ATP-TCA can play a guiding role in individual chemotherapy for human cardia cancer.