多种造血细胞因子在人类羊膜间充质干细胞中表达的意义

Study of IL-3 and other hematopoietic cytokines expression of human amnion-derived mesenchymal stem cells and its significance

目的 研究体外培养的人类羊膜间充质干细胞（MSC）细胞因子分泌特征,探讨其在造血中的分子生物学基础.方法 采用RT-PCR方法在mRNA水平上检测人羊膜MSC细胞因子的分泌.结果 体外传代培养的人类羊膜MSC能表达LIF、SCF、M-CSF、G-CSF、GM-CSF、IL-3、IL-6、IL-11等多种重要的造血细胞因子且比骨髓源MSC表达的造血因子更丰富.结论 人类羊膜MSC可以分泌丰富的造血细胞因子,可能在造血支持和提高造血干细胞移植成功率方面发挥重要作用.

Objective To investigate the cytokine spectrum of cultured human amniotic-derived mesenchymal stem cells （A-MSC） for understanding its basis of molecular biology in hematopoietic in vitro.Methods Their hematopoietic cytokines expression was analyzed using RT-PCR in the mRNA level. Results It showed that in vitro subcultured human amniotic-derived mesenchymal stem cells were capable to express many important hematopoietic cytokines such as LIF, SCF, M-CSF, G-CSF, GM-CSF, IL-3, IL-6, IL-11 and so on. Conclusion Production of abundant of hematopoietic cytokines by human amniotic-derived mesenchymal stem cells may be effective for hematopoietic support and HSC transplantation.