脓毒症中第10染色体丢失的磷酸酶基因在内皮细胞中表达的研究

Expression of PTEN in endothelial cells in sepsis

目的研究在脓毒症中第10染色体丢失的磷酸酶基因(PTEN)在内皮细胞中的表达情况。方法 20只雄性SD大鼠随机分为2组,盲肠结扎穿孔组(CLP组)和对照组;48 h后取血液和肺组织样本。用血浆作用于体外培养的脐静脉内皮细胞(HUVEC),并应用免疫组织化学方法检测PTEN的表达状况;用West-ern-blot的方法检测脐静脉内皮细胞中PTEN蛋白的表达。结果在CLP组脐静脉内皮细胞中PTEN的表达明显增高;CLP组PTEN的表达为(0.513±0.02),对照组PTEN的表达为(0.162±0.025)。CLP组与对照组相比差异有显著性(P<0.01)。结论脓毒症中PTEN基因在HUVEC中表达增高,提示PTEN在脓毒症内皮细胞损伤中发挥了一定的作用。

【Objective】 To investigate the expression of Phosphatase and tensin homolog deleted on chromosome ten（PTEN） in endothelial cells in sepsis.【Methods】 Twenty male Sprague-Dawley rats were randomly divided into the cecal ligation and puncture（CLP） group and the control group.The blood and lung tissues were collected 48 hours after operation.The human umbilical vein endothelial cells（HUVECs） in vitro were stimulated by plasma,the protein expression of PTEN was determined by immunohistochemistry;the protein level of PTEN was examined by Western blot.【Results】 The expression of PTEN in the CLP group was apparently elevated.The content of PTEN was（0.513±0.02） in CLP group and（0.162±0.025） in the control group.There was a significant difference between them（P 〈0.01）.【Conclusion】 The expression of PTEN is elevated in HUVECs in sepsis,which indicates PTEN plays a certain role in endothelial cell injury in sepsis.