IMP3在食管鳞癌中高表达是独立预后因素

Overexpression of IMP3 is an independent prognostic factor for esophageal squamous cell carcinoma

目的研究胰岛素样生长因子ⅡmRNA结合蛋白3(IMP3)在食管鳞癌组织中的表达及其与临床因素之间的关系。方法免疫组织化学检测100对食管鳞癌及正常食管黏膜中的IMP3蛋白表达水平。Realtime PCR检测33对食管鳞癌组织中IMP3的mRNA表达水平。结果 IMP3在癌组织中的表达显著高于癌旁正常组织(17%vs 2%,P<0.001)。IMP3的高表达与食管癌患者预后差之间显著相关(P=0.015),是独立的预后因素。IMP3的mRNA在癌组织中表达升高,但与蛋白水平之间没有相关性。结论 IMP3是食管癌患者预后因素之一。

【Objective】 To investigate the expression of insulin-like growth factor II mRNA binding protein 3（IMP3） and its relationship with clinical characteristics in esophageal squamous cell carcinoma（ESCC）.【Methods】 Immunohistochemistry was performed to detect the expression level of IMP3 protein in tumor tissues and adjacent normal esophageal mucosa from 100 ESCC patients.Quantitive real time PCR was performed to detect the expression level of IMP 3 mRNA in 33 pairs of ESCC tissues.【Results】 The expression level of IMP3 protein was significantly up-regulated in ESCC tissues compared with that in the adjacent normal tissues（17% vs 2%,P 〈0.001）.The overexpression of IMP3 was significantly correlated with poor prognosis of ESCC patients（P =0.015）.Multivariate Cox proportional hazard regression analysis suggested IMP3 as an independent prognostic factor for ESCC patients.The mRNA level of IMP3 was also increased in ESCC tissues,but there was no correlation with protein level.【Conclusion】 IMP3 is up-regulated and functions as one of prognostic factors in ESCC patients.