TRPM8基因表达在前列腺癌中的意义

High expression of TRPM8 in prostate cancer

目的检测TRPM8基因在前列腺癌及前列腺癌旁组织中的表达,探讨TRPM8基因与前列腺癌及前列腺癌旁组织的临床意义。方法运用实时荧光定量PCR方法检测35例前列腺癌病理组织以及42例前列腺癌旁组织的TRPM8的基因表达水平。结果 TRPM8在前列腺癌组织中表达值为(7.732±0.512),在45例前列腺癌旁组织中表达值为(6.623±0.503),前列腺癌中TRPM表达值高于前列腺癌旁组织,差异有显著性(P<0.05)。结论前列腺癌组织中TRPM基因高表达值有助于前列腺癌的诊断。

【Objective】 To study the TRPM8 mRNA expression in PCa tissues and their corresponding non-cancerous tissues,and to explore the value of TRPM8 mRNA expression levels in PCa diagnosis.【Methods】 A sensitive,real-time quantitative PCR assay（QRT-PCR） was developed to detect the expression difference between TRPM8 mRNA in PCa and their corresponding non-cancerous tissues by testing 35 samples of PCa and 42 samples of their corresponding non-cancerous tissues.【Results】 The expression levels of TRPM8 in Pca and non-cancerous tissues were（7.732±0.512） and（6.623±0.503） respectively.The expression level of TRPM8 in Pca was significantly higher than that in non-cancerous tissues（P 〈0.05）.【Conclusion】 The detection result of TRPM8 mRNA expression by QRT-PCR can afford reliable and helpable information for diagnosis of PCa.