肠道病毒71型福建分离株(FJ08089)全基因组序列分析

Complete Genome Analysis of Enterovirus 71 (FJ08089) Strain Isolated in Fujian

[目的]分析2008年福建省手足口病患儿标本肠道病毒71型(EV71)分离株FJ08089全基因组序列特征。[方法]应用RD细胞,从患者疱疹液标本分离EV71,用RT-PCR方法扩增分离株病毒的全基因组,分析其基因组特征。[结果]FJ08089株的基因组长度为7 404 bp,其中包括5′端非编码区(5′UTR)长743 bp,病毒基因组编码区(ORF)全长6 576个核苷酸及3′端非编码区(3′UTR)长81 bp。ORF编码含2 192个氨基酸残基的多聚蛋白。[结论]FJ08089株基因组的结构与安徽地方株ANHUI703814十分相近,整个基因组的核苷酸同源性为93.8%,氨基酸同源性为92.6%,均属于C4亚型,而与Cox.A16国际标准株G10(U05876)差别较大,两者核苷酸同源性仅为77.9%,氨基酸同源性为84.0%。

[Objective] To analyze the complete nucleotide sequence of enterovirus 71（EV71） strain（FJ08089） isolated in Fujian（2008）.[Methods] The FJ08089 whole genomes were amplified by RT-PCR and sequences were analyzed.[Results] The full genomic length of FJ08089 was 7 404 bp,which contained 5′UTR（743 bp）,the open reading frame（ORF,6 576 bp） and 3′UTR（81 bp）.The ORF encoded a 2 192-amino acid residues polyprotein.[Conclusions] Sequence analysis revealed that FJ08089 was closed to ANHUI703814,and the nucleotide and amino acid sequence identities were 93.8% and 92.6% respectively,belonging to C4 sub-genotype.FJ08089 shared lower homology with Cox.A16,the nucleotide and amino acid sequence identities were 77.9% and 84.0% respectively.