温莪术药材RP-HPLC指纹图谱研究

Fingerprints of Curcuma wenyujin by RP-HPLC

目的:建立温莪术药材RP-HPLC指纹图谱的共有模式,控制温莪术药材质量。方法:采用Waters Sym-metry C18色谱柱;流动相为甲醇-水,梯度洗脱;流速:1.0 mL/min;柱温:30℃;检测波长:215 nm。测定10批不同产地不同采集时间的温莪术药材的RP-HPLC指纹图谱,并在相同色谱条件下对3批蓬莪术、3批广西莪术进行测定。结果:10批温莪术药材得到的指纹图谱共有11个共有峰,其中S峰为=牛儿酮,在相同色谱条件下3种莪术药材的HPLC色谱图存在很大差异。结论:温莪术药材HPLC指纹图谱特征性及专属性强,可结合含量测定用于温莪术药材的质量控制。

Objective：To establish a mutual mode fingerprint of Curcuma wenyujin for quality control of C.wenyujin.Methods：Waters Symmetry C18 column was used;the mobile phase was methanol-water in a linear gradient elution with the flow rate of 1.0 mL/min,the temperature of column was 30 ℃;the detection wavelength was set at 215 nm.10 batches of C.wenyujin from different places and different time were determined,3 batches of C.phaeocaulis and 3 batches of C.kwangsiensis were determined in the same chromatographic conditions.Results：11 mutual peaks in the fingerprint of the 10 groups of C.wenyujin,and the S peak among them represented germacrone.Conclusion：Curcuma wenyujin′s fingerprints have strong feature and specificity,which can be combined with assaying in the quality control of C.wenyujin.