六味地黄丸4种入血成分组合对大鼠前脂肪细胞增殖、分化影响的最佳浓度配伍研究

Study on the Best Concentration of Combination of the Effects of the Four Original Constituents of Liu-wei Di-huang Pill on the Proliferation and Differentation of Rat Preadipocyte

目的:探讨六味地黄丸中马钱苷、芍药苷、莫诺苷、丹皮酚4种入血成分组合对大鼠前脂肪细胞增殖、分化的影响,并优选其最佳浓度配伍。方法:原代培养大鼠的前脂肪细胞,将马钱苷等4种入血成分按四因素三水平的正交试验表L9(34)安排实验,同时设立空白对照组,以四甲基偶氮唑盐(MTT)法测定细胞增殖的速度,以油红O染色方法检测其对大鼠前脂肪细胞分化过程中的细胞内脂肪积聚的影响,以光密度为指标比较各因素、水平之间的差异。结果:六味地黄丸4种入血成分组合对大鼠前脂肪细胞增殖、分化的影响以组合A3B1C3D2的作用最为显著(P<0.01);与各单体组分作用比较,具有高度显著性差异(P<0.01)。结论:六味地黄丸4种入血成分以马钱苷、莫诺苷高浓度,丹皮酚中浓度,芍药苷低浓度配伍组合对大鼠前脂肪细胞增殖、分化影响较单体组分作用显著,表现为中药组方的整体性和协同增效调节作用。

Objective：To investigate the effects of four original constituents（Loganin,Morroniside,Peoniflorin,Paeonol） of Liu-wei Di-huang pills on the proliferation and differentiation of rat preadipocyte,and optimize the best concentration by the orthogonal test.Methods： The rat preadipocytes were cultured,then above four original constituents according to 4 factors and 3 levels to make L9（34） orthogonal test,and the control was performed at the same time.The proliferation of preadipocytes was determined by MTT method,and the accumulation of cellular lipid was determined by Oil Red O staining,the differences between factors and variances were compared by the value of absorbance.Results： The combination of four original constituents of Liu-wei Di-huang pills could stimulate rat preadipocyte proliferation and inhibited its lipid accumulation.Both The effects were highly significant（P0.01） with the combination of A3B1C3D2;Compared with the monosomic group,the effects of the A3B1C3D2 was highly significant（P0.01）.Conclusion： Compared with the monosomic group,the effects of the combination of the four original constituents of Liu-wei Di-huang pills on the proliferation and differentation of rat preadipocyte were highly significant.This combination consists of Loganin,morroniside with high doses,Paeonol,Peoniflorin with mid-dose and low-dose,respectively.Regnesent the ouerall regulatory role of synergy and officiencg.