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胶质瘤U251细胞化疗过程中MGMT表达改变与耐药的关系

Expression of MGMT and Chemo-resistance during U251 Glioma Cells' Chemotherapy
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摘要 目的探讨胶质瘤U251细胞中MGMT表达与卡莫司汀(BCNU)和替莫唑胺(TMZ)化疗耐药的关系。方法细胞生长情况用MTT进行评估,细胞周期采用流式细胞仪检测,MGMT表达用western-blot检测。结果 U251细胞对BCNU和TMZ存在耐药现象。在化疗过程中,处于G0/G1期细胞比例明显增加,MGMT表达无显著变化。结论利用BCNU和TMZ对U251进行化疗过程中,不能通过消耗MGMT,来达到逆转耐药现象的目的 。 Objective To approach the relation between expression of MGMT and carmustine and temozolomide chemo-resistance during U251 glioma cells＇ chemotherapy.Methods Cell growth was assessed by MTT and cycle was detected by flow cytometry.Expression of MGMT was determined by western-blot.Results U251 glioma cells were resistance to BCNU and TMZ.During chemotherapy,the ratio of G0/G1 phrase was increased obviously while the expression of MGMT had no significant change.Conclusion The chemo-resistance during U251 glioma cells＇ chemotherapy by BCNU and TMZ can not be reversed by depleting MGMT.
作者 吴小明 仇有成 王存祖 欧阳琦 黄钱焕 杨伟现 许慧中
机构地区 江苏省宜兴市人民医院神经外科 江苏大学附属医院神经外科 江苏大学基础医学与医学技术学院 江苏省江阴市人民医院急诊科
出处 《医药论坛杂志》 2010年第22期16-18,21,共4页 Journal of Medical Forum
基金 江苏省卫生厅面上科研项目资助(H2006040)
关键词 胶质瘤 O6-甲基鸟嘌呤-DNA-甲基转酶(MGMT) 化疗耐药 Glioma O6-methylguanine methyltransferase（MGMT） Chemo-resistance
分类号 R730.264 [医药卫生—肿瘤]
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参考文献5

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二级参考文献10

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医药论坛杂志
2010年 第22期

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